Expression of Diverse Angiogenesis Factor in Different Stages of the 4T1 Tumor as a Mouse Model of Triple-Negative Breast Cancer
| Autor: | Monireh Kazemimanesh, Soyar Sari, Saeid Amanpour, Saba Malekian, Ahad Muhammadnejad, Marveh Rahmati, Raheleh Kheirbakhsh |
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| Rok vydání: | 2020 |
| Předmět: |
CD31
Angiogenesis Pharmaceutical Science Angiogenesis factor Metastasis 03 medical and health sciences 0302 clinical medicine Breast cancer Vascularity Triple-negative breast cancer Medicine General Pharmacology Toxicology and Pharmaceutics Stage (cooking) 030304 developmental biology 0303 health sciences business.industry lcsh:RM1-950 medicine.disease VEGF lcsh:Therapeutics. Pharmacology VEGFR2 bFGF VEGFR1 030220 oncology & carcinogenesis Cancer research Immunohistochemistry medicine.symptom business Research Article |
| Zdroj: | Advanced Pharmaceutical Bulletin Advanced Pharmaceutical Bulletin, Vol 10, Iss 2, Pp 323-328 (2020) |
| ISSN: | 2251-7308 2228-5881 |
| DOI: | 10.34172/apb.2020.039 |
| Popis: | Purpose: Triple-negative breast cancer (TNBC) is specified by high vascularity and repetitious metastasis. Although several studies have indicated that angiogenesis has an important role in invasive breast cancer, a suitable model of TNBC that can show the exact onset of angiogenesis factors still needs to be developed. The purpose of this study is to determine the expression level of angiogenesis factors in different clinical stages of the 4T1 tumor as TNBC mouse model. Methods: Twenty mice were injected by the 4T1 cell line, and four mice selected as healthy controls. Following by tumor induction, the mice were randomly put into four groups, each contains four mice. Once the tumor volume reached to the early stage (500 mm3 ), they were removed by surgery. Then, the expression levels of Hif1α, VEGFR1, and VEGFR2 genes, as well as tumor markers of VEGF, bFGF and CD31, were evaluated by qPCR and immunohistochemistry (IHC) respectively. The statistical analysis was done by SPSS version 16. Results: TNBC tumors were confirmed and multi-foci metastasis in the lung were seen. The mRNA and protein expression levels of the angiogenesis factors increased in the early stage and as the tumor grew, their expression level enhanced dramatically. Conclusion: The 4T1 syngeneic mouse tumor may serve as an appropriate TNBC model for further investigation of the angiogenesis and therapies. Moreover, angiogenesis factors are induced before the advanced stage, and anti-angiogenesis therapy is necessary to be considered at the first line of treatment in TBNC. |
| Databáze: | OpenAIRE |
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