Relationship of CD44+CD24-/low breast cancer stem cells and axillary lymph node metastasis
| Autor: | Adrian Y S Yip, Louis W.C. Chow, Haosheng Tan, Wings T.Y. Loo, Hui Hu, Wei Wei |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Oncology
Adult medicine.medical_specialty Axillary lymph nodes Breast Neoplasms General Biochemistry Genetics and Molecular Biology Metastasis Young Adult Breast cancer Internal medicine medicine Humans skin and connective tissue diseases Lymph node Aged Medicine(all) biology Staining and Labeling CD24 business.industry Biochemistry Genetics and Molecular Biology(all) CD44 CD24 Antigen General Medicine Middle Aged medicine.disease Axilla medicine.anatomical_structure Proceedings Hyaluronan Receptors Lymphatic Metastasis biology.protein Neoplastic Stem Cells Female Lymph Nodes Stem cell business |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| DOI: | 10.1186/1479-5876-10-s1-s6 |
| Popis: | Background Axillary node staging plays an important role in the prognostic evaluation and planning of adjuvant treatment. Breast cancer stem cells, identified on the basis of CD44+CD24-/low expression, are associated with metastases and drug resistance. It is therefore important to investigate the proportion of CD44+CD24-/low breast cancer stem cells for the diagnosis of metastases in axillary nodes. Methods Thirty-two ipsilateral axillary lymph nodes were collected from patients diagnosed with invasive breast cancer. Each lymph node (LN) was divided into two equals – one was examined by H&E staining, while the other was made into a single cell suspension to study the content of CD44+CD24-/low cells by flow cytometry (FCM). The relationship was investigated between the content of CD44+CD24-/low cells and metastases in axillary nodes which were confirmed by histology. Associations were tested using the chi-square test (linear-by-linear association), and the significance level was set at a value of p < 0.05. Results In the 32 axillary nodes, the level of CD44+CD24-/low cells was determined to be between 0 and 18.4%: there was no presence of CD44+CD24-/low cells in 9 LNs, of which 2 had confirmed metastasis; there were less than 10% CD44+CD24-/low cells in 12 LNs, of which 6 had confirmed metastasis; and there were more than 10% CD44+CD24-/low cells in 11 LNs, of which 9 had confirmed metastasis. A higher percentage of detected CD44+CD24-/low cells was significantly associated with more confirmed LN metastases (p = 0.009). Conclusions CD44+CD24-/low breast cancer stem cells might help clinicians to determine the presence of LN metastases. However, its prognostic value remains unclear, while histological diagnosis is still the gold standard. |
| Databáze: | OpenAIRE |
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