Photothermal detection of individual gold nanoparticles: perspectives for high-throughput screening

Autor: Michel Orrit, Herman P. Spaink, Thomas Schmidt, Niels Laurens, Jaya Besser, Florian Kulzer
Přispěvatelé: Physics of Living Systems
Rok vydání: 2008
Předmět:
Zdroj: Kulzer, F, Laurens, N, Besser, J, Schmidt, T, Orrit, M & Spaink, H P 2008, ' Photothermal detection of individual gold nanoparticles: Perspectives for high-throughput screening ', ChemPhysChem, vol. 9, no. 12, pp. 1761-1766 . https://doi.org/10.1002/cphc.200800127
ChemPhysChem, 9(12), 1761-1766. Wiley-VCH Verlag
ISSN: 1439-7641
1439-4235
Popis: We use photothermal microscopy to detect and image individual gold nanoparticles that are either embedded in a polymer film or immobilized in an aqueous environment. Reducing the numerical aperture of the detection optics allows us to achieve a 200-fold-enlarged detection volume while still retaining sufficient detectivity. We characterize the capabilities of this approach for the detection of gold colloids with a diameter of 20 nm, with emphasis on practical aspects that are important for high-throughput-screening applications. The extended detection volume in combination with the stability of the photothermal signal are major advantages compared to fluorescence-based approaches, which are limited by photoblinking and photobleaching. Careful consideration is given to the trade-off between the maximum increase in local temperature that can be tolerated by a biological specimen and the minimum integration time needed to reliably determine whether a given volume contains a target species. We find that our approach has the potential to increase the detection-limited flow rate (i.e. the limit given by the detection volume divided by the minimum detection time) by two to three orders of magnitude.
Databáze: OpenAIRE
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