The RU5 (‘R’) region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence
| Autor: | Philippe Bolifraud, Louis-Marie Houdebine, Marco Cajero-Juarez, Marie-Claire Théron, Guy Kann, Joe Attal, Frédérique Taboit |
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| Přispěvatelé: | Unité de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Předmět: |
Untranslated region
Genes Viral Picornavirus viruses Simian virus 40 Biochemistry Mice Structural Biology Cricetinae Leukemia Virus Bovine Protein biosynthesis Luciferases Cells Cultured ComputingMilieux_MISCELLANEOUS Human T-lymphotropic virus 1 0303 health sciences biology 030302 biochemistry & molecular biology Human T cell leukaemia virus 1 Translation (biology) Recombinant Proteins 3. Good health RNA Viral Internal ribosome entry site Genetic Vectors Molecular Sequence Data Biophysics CHO Cells [SDV.BC]Life Sciences [q-bio]/Cellular Biology Transfection 03 medical and health sciences Genetics Animals Binding site [SDV.BC] Life Sciences [q-bio]/Cellular Biology Molecular Biology Gene 030304 developmental biology Binding Sites Base Sequence fungi Bovine leukaemia virus RNA Cell Biology R region biology.organism_classification Virology Molecular biology Growth Hormone Protein Biosynthesis Nucleic Acid Conformation Ribosomes |
| Zdroj: | FEBS Letters FEBS Letters, Wiley, 1996, 392, pp.220-224 |
| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/0014-5793(96)00815-0 |
| Popis: | RNA fragments containing the complete R region and the beginning of the U5 region (‘R’) from the human T cell leukaemia virus 1 (HTLV-1) stimulated the translation of the second cistrons in bicistronic mRNAs. The 5′ untranslated region from SV40 early genes (SU) which was unable to stimulate translation of second cistrons amplified markedly the internal ribosome entry site (IRES) effect of the HTLV-1 ‘R’ fragments. The ‘R’ regions from HTLV-1 have therefore properties similar to internal ribosome entry sites (IRES) originally found in picornavirus. The beginning of the U5 region from HTLV-1 contains a polypyrimidine sequence which is known to play an essential role in the IRES activity in picornavirus. The same experiments carried out using the ‘R’ region from bovine leukaemia virus (BLV) showed that this sequence has at most a weak IRES effect. One retroviruses HTLV-1 and perhaps others contain therefore an IRES activity. Interestingly, the combined SU ‘R’ sequence worked efficiently with different cistrons, different promoters and in all tested cell lines, whereas the poliovirus IRES was active in CHO cells but not in the mouse mammary cell line HC11. The SU ‘R’ sequence may therefore preferably be used to generate active bicistronic mRNAs. |
| Databáze: | OpenAIRE |
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