Alteration of adenoid tissue alkaline and acid phosphatase in children with secretory otitis media

Autor: Vladimir Nesic, Svetlana Stosic, Vladimir Djordjevic, Zeljko Petrovic, Ljuba Stojiljkovic, Snezana Jesic
Rok vydání: 2006
Předmět:
Male
Pathology
Palatine Tonsil
Tonsillitis
Palatine tonsil
Adenoidectomy
Nitrophenols
0302 clinical medicine
Recurrence
Child
B-Lymphocytes
0303 health sciences
biology
General Medicine
Hydrogen-Ion Concentration
respiratory system
3. Good health
medicine.anatomical_structure
Child
Preschool
030220 oncology & carcinogenesis
Alkaline phosphatase
Female
Nasal Obstruction
medicine.symptom
medicine.medical_specialty
Acid Phosphatase
Phosphatase
Adenoid
03 medical and health sciences
Organophosphorus Compounds
stomatognathic system
otorhinolaryngologic diseases
medicine
Humans
Tonsillectomy
030304 developmental biology
Otitis Media with Effusion
business.industry
Macrophages
Acid phosphatase
Alkaline Phosphatase
medicine.disease
stomatognathic diseases
Otitis
Otorhinolaryngology
Tonsil
Adenoids
Pediatrics
Perinatology and Child Health
Immunology
biology.protein
Indicators and Reagents
business
Dendritic Cells
Follicular
Zdroj: International Journal of Pediatric Otorhinolaryngology. 70:1069-1076
ISSN: 0165-5876
Popis: The role of pharyngeal lymphoid tissue in etiopathogenesis of secretory otitis is not yet defined. The influence of tonsillar and adenoid mass, weight, obstruction of naspharyngeal orrifitium, bacterial reservoire or some immunological events are of scientific interest. Tissue nonspecific alkaline phosphatase (TNAP) and acid phosphatase (ACP) are enzymes detected in lymphoid tissue, TNAP as characteristic of B cells, ACP as a characteristic of macrophages and folucullardentritic cells. These enzymes interfere in cell metabolism by removing 5' phosphate group from nucleotides and proteins. Specific activity and kinetic properties were studied in palatinal tonsils and adenoids of children with secretory otitis (OME) and compared with children with recurrent tonsillitis without ear involvement.Adenoid and tonsillar tissue of l7 children with OME and 30 children with recurrent tonsillitis were subjected to biochemical investigation using method of releasing of p-nitrophenol from p-nitrophenylphosphate (pNPP). Kinetic parameters as Michaelis-Menten constant were calculated by non-linear regression estimation method.Specific activity of adenoid alkaline phosphatase was lower in children with OME in relation to children with recurrent tonsillitis (t=5.733507, p0.01). Specific activity of adenoid acid phosphatase was also lower in children with OME (t=3.655456, p0.01). pH optimum for both enzymes was the same in these two groups of children. Michaelis-Menten constant for both enzymes was significantly higher in adenoid of children with OME than in children with recurrent tonsillitis suggesting lower enzyme affinity for the substrate.Differences in specific activities and kinetic properties of adenoid alkaline and acid phosphatases between children with OME and children with recurrent tonsillitis without OME were verified in this study. The results of the study are not able to explain the alteration of alkaline and acid phosphatase characteristics but could point to some possible and specific role of nasopharyngeal lymphoid tissue in pathogenesis of secretary otitis.
Databáze: OpenAIRE
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