Development and characterization of EST-SSR markers in Ostryopsis (Betulaceae)
| Autor: | Zhiqiang Lu, Bingbing Liu, Hui Ma, Kangshan Mao, Bin Tian, Qiang Qiu, Jianquan Liu |
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| Rok vydání: | 2013 |
| Předmět: |
Genetics
Expressed sequence tag Genetic diversity biology Locus (genetics) Plant Science Ostryopsis biology.organism_classification Primer Note Gene flow lcsh:QK1-989 Genetic divergence Loss of heterozygosity lcsh:Biology (General) Betulaceae lcsh:Botany Microsatellite next-generation sequencing technology lcsh:QH301-705.5 ortholog genes Ecology Evolution Behavior and Systematics EST-SSR marker |
| Zdroj: | Applications in Plant Sciences Applications in Plant Sciences, Vol 2, Iss 2, p 1300062 (2014) |
| ISSN: | 2168-0450 |
| Popis: | Premise of the study: A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus Ostryopsis (Betulaceae). Methods and Results: A total of 38 high-quality simple sequence repeat (SSR) primers were identified, of which 15 could be successfully amplified. Subsequently, we selected 80 individuals to represent the three species of the genus to evaluate the efficacy of these markers for examining genetic diversity of each species in the future. We found that the number of alleles per locus ranged from one to nine, with an average of 3.8. The expected heterozygosity and observed heterozygosity per locus varied from 0 to 0.829 and from 0 to 1, respectively, with their respective mean values as 0.483 and 0.416. Conclusions: These EST-SSR markers will be useful for evaluating the range-wide genetic diversity of each species and examining genetic divergence and gene flow between the three species. |
| Databáze: | OpenAIRE |
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