A developmentally regulated two-step process generates a noncentrosomal microtubule network in Drosophila tracheal cells
| Autor: | Antoine Guichet, Pierre-Marie Le Droguen, Véronique Brodu, Alexandre Baffet, Jordi Casanova |
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| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut de Recerca Biomèdica de Barcelona, Parc Cientific de Barcelona |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Cell Differentiation
MESH: Cell Nucleus Cell type Embryo Nonmammalian MESH: Drosophila MESH: Drosophila Proteins [SDV]Life Sciences [q-bio] Morphogenesis Motility MESH: Carrier Proteins Biology Spastin MESH: Centrosome Microtubules General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Microtubule MESH: Adenosine Triphosphatases Animals Drosophila Proteins MESH: Animals Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology 030304 developmental biology Centrioles Adenosine Triphosphatases Cell Nucleus Centrosome 0303 health sciences MESH: Microtubules MESH: Centrioles MESH: Embryo Nonmammalian Microtubule organizing center Cell Differentiation Cell Biology Transmembrane protein MESH: Morphogenesis Cell biology Trachea MESH: Microtubule-Associated Proteins Drosophila Carrier Proteins Microtubule-Associated Proteins 030217 neurology & neurosurgery MESH: Trachea Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2010, 18 (5), pp.790-801. ⟨10.1016/j.devcel.2010.03.015⟩ Developmental Cell, Elsevier, 2010, 18, pp.790-801. ⟨10.1016/j.devcel.2010.03.015⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1878-1551 1534-5807 |
| DOI: | 10.1016/j.devcel.2010.03.015⟩ |
| Popis: | Microtubules (MTs) are essential for many cell features, such as polarity, motility, shape, and vesicle trafficking. Therefore, in a multicellular organism, their organization differs between cell types and during development; however, the control of this process remains elusive. Here, we show that during Drosophila tracheal morphogenesis, MT reorganization is coupled to relocalization of the microtubule organizing centers (MTOC) components from the centrosome to the apical cell domain from where MTs then grow. We reveal that this process is controlled by the trachealess patterning gene in a two-step mechanism. MTOC components are first released from the centrosome by the activity of the MT-severing protein Spastin, and then anchored apically through the transmembrane protein Piopio. We further show that these changes are essential for tracheal development, thus stressing the functional relevance of MT reorganization for morphogenesis. © 2010 Elsevier Inc. Este trabajo ha sido financiado por las conceciones del CNRS y ANR « Blanche » (subvención Cymempol, Blan06-3-139786). Jordi Casanova ha sido financiado por la Generalitat de Catalunya, el Ministerio de España de Ciencia e Innovación, y su programa Consolider-Ingenio 2010. |
| Databáze: | OpenAIRE |
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