Dynamics of the bacterial gut microbiota during controlled human infection with Necator americanus larvae
| Autor: | Jelle J. Goeman, Ed J. Kuijper, A. R. Geelen, Jacqueline J. Janse, Quinten R Ducarmon, Romy D. Zwittink, Marie-Astrid Hoogerwerf, Jan Pieter R. Koopman, Meta Roestenberg |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) longitudinal Anemia Necator americanus animal diseases chemical and pharmacologic phenomena Gut microbiota Gut flora Microbiology 03 medical and health sciences 0302 clinical medicine Immune system parasitic diseases medicine Helminths lcsh:RC799-869 helminth Larva biology Gastroenterology biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases medicine.anatomical_structure Duodenum bacteria lcsh:Diseases of the digestive system. Gastroenterology 030211 gastroenterology & hepatology controlled human infection hookworm |
| Zdroj: | GUT MICROBES, 12(1). TAYLOR & FRANCIS INC GUT MICROBES Gut Microbes, Vol 12, Iss 1 (2020) |
| Popis: | Hookworms are soil-transmitted helminths that use immune-evasive strategies to persist in the human duodenum where they are responsible for anemia and protein loss. Given their location and immune regulatory effects, hookworms likely impact the bacterial microbiota. However, microbiota studies struggle to deconvolute the effect of hookworms from confounders such as coinfections and malnutrition. We thus used an experimental human hookworm infection model to explore temporal changes in the gut microbiota before and during hookworm infection. Volunteers were dermally exposed to cumulative dosages of 50, 100 or 150 L3 Necator americanus larvae. Fecal samples were collected for microbiota profiling through 16S rRNA gene amplicon sequencing at weeks zero, four, eight, fourteen and twenty. During the acute infection phase (trial week zero to eight) no changes in bacterial diversity were detected. During the established infection phase (trial week eight to twenty), bacterial richness (Chao1, p = .0174) increased significantly over all volunteers. No relation was found between larval dosage and diversity, stability or relative abundance of individual bacterial taxa. GI symptoms were associated with an unstable microbiota during the first eight weeks and rapid recovery at week twenty. Barnesiella, amongst other taxa, was more abundant in volunteers with more GI symptoms throughout the study. In conclusion, this study showed that clinical GI symptoms following N. americanus infection are associated with temporary microbiota instability and relative abundance of specific bacterial taxa. These results suggest a possible role of hookworm-induced enteritis on microbiota stability. |
| Databáze: | OpenAIRE |
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