Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach
| Autor: | Sébastien Dutertre, Fabien Pierrat, Reto Stöcklin, David Piquemal, Aude Violette, Daniel Biass, Dominique Koua, Philippe Favreau |
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| Přispěvatelé: | Immunologie et chimie thérapeutiques (ICT), Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Skuldtech, Skuld-Tech, biotechnology company (Skuld-Tech), Skuld-Tech, Atheris Laboratories, Laboratoire de neurobiologie cellulaire et moléculaire (NBCM), Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Protein family [SDV]Life Sciences [q-bio] Molecular Sequence Data Biophysics Mollusk Venoms Venom Computational biology complex mixtures Biochemistry Injections Cone snail 03 medical and health sciences Drug Discovery Conus Animals Combinatorial Chemistry Techniques Amino Acid Sequence Conotoxin 030304 developmental biology 0303 health sciences Sequence Homology Amino Acid Molecular mass biology Drug discovery Gene Expression Profiling 030302 biochemistry & molecular biology Conus Snail Proteins Anatomy biology.organism_classification High-Throughput Screening Assays Conus consors Conotoxins Peptides Transcriptome |
| Zdroj: | Journal of Proteomics Journal of Proteomics, Elsevier, 2012, 75 (17), pp.5215-5225. ⟨10.1016/j.jprot.2012.06.001⟩ |
| ISSN: | 1874-3919 1876-7737 |
| DOI: | 10.1016/j.jprot.2012.06.001 |
| Popis: | International audience; Predatory marine snails of the genus Conus use venom containing a complex mixture of bioactive peptides to subdue their prey. Here we report on a comprehensive analysis of the protein content of injectable venom from Conus consors, an indo-pacific fish-hunting cone snail. By matching MS/MS data against an extensive set of venom gland transcriptomic mRNA sequences, we identified 105 components out of ~400 molecular masses detected in the venom. Among them, we described new conotoxins belonging to the A, M- and O1-superfamilies as well as a novel superfamily of disulphide free conopeptides. A high proportion of the deduced sequences (36%) corresponded to propeptide regions of the A- and M-superfamilies, raising the question of their putative role in injectable venom. Enzymatic digestion of higher molecular mass components allowed the identification of new conkunitzins (~7 kDa) and two proteins in the 25 and 50 kDa molecular mass ranges respectively characterised as actinoporin-like and hyaluronidase-like protein. These results provide the most exhaustive and accurate proteomic overview of an injectable cone snail venom to date, and delineate the major protein families present in the delivered venom. This study demonstrates the feasibility of this analytical approach and paves the way for transcriptomics-assisted strategies in drug discovery. |
| Databáze: | OpenAIRE |
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