Qualitative and Quantitative Analyses of Glycogen in Human Milk
| Autor: | Takashi Furuyashiki, Takashi Kuriki, Miyuki Ishida, Hiroshi Kamasaka, Hiroko Matsui-Yatsuhashi, Hiroki Takata, Ryo Kakutani, Junko Hirose, Saeko Nagao, Hiroko Takumi |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty Biology Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound fluids and secretions Qualitative analysis Internal medicine medicine Humans Food science Milk Human Glycogen food and beverages General Chemistry medicine.disease Mastitis 030104 developmental biology Endocrinology chemistry Female General Agricultural and Biological Sciences Quantitative analysis (chemistry) |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:1314-1319 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.6b03644 |
| Popis: | Identification as well as a detailed analysis of glycogen in human milk has not been shown yet. The present study confirmed that glycogen is contained in human milk by qualitative and quantitative analyses. High-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography with a multiangle laser light scattering detector (HPSEC-MALLS) were used for qualitative analysis of glycogen in human milk. Quantitative analysis was carried out by using samples obtained from the individual milks. The result revealed that the concentration of human milk glycogen varied depending on the mother's condition-such as the period postpartum and inflammation. The amounts of glycogen in human milk collected at 0 and 1-2 months postpartum were higher than in milk collected at 3-14 months postpartum. In the milk from mothers with severe mastitis, the concentration of glycogen was about 40 times higher than that in normal milk. |
| Databáze: | OpenAIRE |
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