The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets
| Autor: | Shunsaku Sugiura, Naomichi Nishimura, Shingo Hino, Tomomi Genda, Takashi Kondo, Tatsuya Morita |
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| Rok vydání: | 2018 |
| Předmět: |
Chromium
Male 0301 basic medicine Succinic Acid Oligosaccharides Medicine (miscellaneous) Fructose Bacterial translocation digestive system Permeability Excretion 03 medical and health sciences Cecum medicine Gut permeability Animals Mesenteric lymph nodes Lactic Acid Intestinal Mucosa Rats Wistar Gastrointestinal Transit Edetic Acid Peroxidase Inflammation 030109 nutrition & dietetics Nutrition and Dietetics Chemistry Fatty Acids Volatile Animal Feed Molecular biology Diet Gastrointestinal Microbiome Cecal mucosa Prebiotics 030104 developmental biology medicine.anatomical_structure Bacterial Translocation Fermentation Cytokines Digestion Animal studies |
| Zdroj: | Journal of Nutritional Science and Vitaminology. 64:357-366 |
| ISSN: | 1881-7742 0301-4800 |
| DOI: | 10.3177/jnsv.64.357 |
| Popis: | The effects of fructo-oligosaccharides (FOS) on gut-barrier function are still controversial in human and animal studies. Diet conditions would be a major factor for the controversy in animal studies. We fed rats a semi-purified (SP) or a non-purified diet (NP) with or without FOS (60 g/kg diet) for 9 (experiment 1) or 10 d (experiment 2). We assessed microbial fermentation, gut permeability, and inflammatory responses in the cecum (experiment 1), and mucus layer in the cecum, intestinal transit time and microbiota composition (experiment 2). FOS supplementation induced a very acidic fermentation due to the accumulation of lactate and succinate in SP, while short-chain fatty acids were major products in NP. Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation into mesenteric lymph nodes, myeloperoxidase activity, and expressions of the inflammatory cytokine genes in the cecal mucosa were greater in SP+FOS than in SP, but these alterations were not observed between NP and NP+FOS (experiment 1). FOS supplementation destroyed the mucus layer on the epithelial surface in SP, but not in NP. Intestinal transit time was 3-fold longer in SP+FOS than in SP, but this was not the case between NP and NP+FOS. Lower species richness of cecal microbiota was manifest solely in SP+FOS (experiment 2). These factors suggest that impact of FOS on gut permeability and inflammatory responses in the cecal mucosa quite differs between SP and NP. Increased gut permeability in SP+FOS could be evoked by the disruption of the mucus layer due to stasis of the very acidic luminal contents. |
| Databáze: | OpenAIRE |
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