Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
| Autor: | Er-Fu Zhang, Ting Xue, Zheng-Ming Fang, Chun-Li An, Li He |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
China
Zoology Spirometra erinaceieuropaei genetic analysis macromolecular substances Brief Communication Polymerase Chain Reaction Genetic analysis law.invention law Genetic variation Animals Humans Cox1 gene Spirometra Phylogeny Polymerase chain reaction Direct sequencing biology Helminth Proteins spargana Cestode Infections biology.organism_classification cox1 genomic DNA Infectious Diseases Cyclooxygenase 1 Parasitology Anura Reference genome |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces. |
| Databáze: | OpenAIRE |
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