Integrated Portable Genetic Analysis Microsystem for Pathogen/Infectious Disease Detection
| Autor: | Eric T. Lagally, James R. Scherer, Richard A. Mathies, Binh An Diep, Lee W. Riley, George F. Sensabaugh, Robert G. Blazej, Nicholas Toriello, M Ramchandani |
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| Rok vydání: | 2004 |
| Předmět: |
Detection limit
Bacteria Base Sequence Serial dilution Chemistry Molecular Sequence Data Electrophoresis Capillary Bacterial Infections Staphylococcal Infections medicine.disease_cause Polymerase Chain Reaction Molecular biology Analytical Chemistry law.invention Capillary electrophoresis law Staphylococcus aureus Microsystem medicine Animals Genotyping Escherichia coli Escherichia coli Infections Polymerase chain reaction Oligonucleotide Array Sequence Analysis |
| Zdroj: | Analytical Chemistry. 76:3162-3170 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac035310p |
| Popis: | An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact instrument for electrical control and laser-excited fluorescence detection has been developed. The microdevice contains microfabricated heaters, temperature sensors, and membrane valves to provide controlled sample positioning and immobilization in 200-nL PCR chambers. The instrument incorporates a solid-state laser and confocal fluorescence detection optics, electronics for sensing and powering the PCR reactor, and high-voltage power supplies for conducting CE separations. The fluorescein-labeled PCR products are amplified and electrophoretically analyzed in a gel-filled microchannel in |
| Databáze: | OpenAIRE |
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