Functional consequences of prolactin signalling in endothelial cells: a potential link with angiogenesis in pathophysiology?
Autor: | Arjan W. Griffioen, Vincent Goffin, Patrycja Nowak-Sliwinska, Chris M. van der Loos, Anne Q Reuwer, Keren Borensztajn, Jan H. von der Thüsen, C. Arnold Spek, Laurie A Mans, Marcel Th. B. Twickler |
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Přispěvatelé: | Other departments, Pathology, Amsterdam Cardiovascular Sciences, Amsterdam institute for Infection and Immunity, Cancer Center Amsterdam, Center of Experimental and Molecular Medicine, Medical oncology laboratory, CCA - Innovative therapy |
Rok vydání: | 2012 |
Předmět: |
prolactin
endocrine system medicine.medical_specialty prolactin receptor MAP Kinase Signaling System Receptors Prolactin Angiogenesis Breast Neoplasms Chick Embryo Biology Cell Line Prolactin cell angiogenesis Mice Internal medicine STAT5 Transcription Factor medicine Animals Phosphorylation Intussusceptive angiogenesis pathophysiology Matrigel Neovascularization Pathologic Prolactin receptor Endothelial Cells Original Articles Cell Biology Immunohistochemistry Prolactin Cell biology Endothelial stem cell Drug Combinations Endocrinology Molecular Medicine Angiogenesis Inducing Agents Female Proteoglycans Human medicine Collagen Laminin hormones hormone substitutes and hormone antagonists Signal Transduction |
Zdroj: | Journal of cellular and molecular medicine, 16(9), 2035-2048. Wiley-Blackwell Reuwer, A Q, Nowak-Sliwinska, P, Mans, L A, van der Loos, C M, von der Thusen, J H, Twickler, M T B, Spek, C A, Goffin, V, Griffioen, A W & Borensztajn, K S 2012, ' Functional consequences of prolactin signalling in endothelial cells: a potential link with angiogenesis in pathophysiology? ', Journal of Cellular and Molecular Medicine, vol. 16, no. 9, pp. 2035-2048 . https://doi.org/10.1111/j.1582-4934.2011.01499.x Journal of Cellular and Molecular Medicine, 16(9), 2035-2048. Wiley-Blackwell Journal of Cellular and Molecular Medicine Journal of cellular and molecular medicine |
ISSN: | 1582-1838 1582-4934 |
Popis: | Prolactin is best known as the polypeptide anterior pituitary hormone, which regulates the development of the mammary gland. However, it became clear over the last decade that prolactin contributes to a broad range of pathologies, including breast cancer. Prolactin is also involved in angiogenesis via the release of pro-angiogenic factors by leukocytes and epithelial cells. However, whether prolactin also influences endothelial cells, and whether there are functional consequences of prolactin-induced signalling in the perspective of angiogenesis, remains so far elusive. In the present study, we show that prolactin induces phosphorylation of ERK1/2 and STAT5 and induces tube formation of endothelial cells on Matrigel. These effects are blocked by a specific prolactin receptor antagonist, del1-9-G129R-hPRL. Moreover, in an in vivo model of the chorioallantoic membrane of the chicken embryo, prolactin enhances vessel density and the tortuosity of the vasculature and pillar formation, which are hallmarks of intussusceptive angiogenesis. Interestingly, while prolactin has only little effect on endothelial cell proliferation, it markedly stimulates endothelial cell migration. Again, migration was reverted by del1-9-G129R-hPRL, indicating a direct effect of prolactin on its receptor. Immunohistochemistry and spectral imaging revealed that the prolactin receptor is present in the microvasculature of human breast carcinoma tissue. Altogether, these results suggest that prolactin may directly stimulate angiogenesis, which could be one of the mechanisms by which prolactin contributes to breast cancer progression, thereby providing a potential tool for intervention. |
Databáze: | OpenAIRE |
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