GABAergic/Glutamatergic–Glial/Neuronal Interaction Contributes to Rapid Adaptation in Pacinian Corpuscles
| Autor: | Adam K. Pack, Burak Güçlü, Laura T. Prestia, Philip J. Cox, Greer K. Mahoney, Lorraine Pawson |
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| Rok vydání: | 2009 |
| Předmět: |
Neurite
Action Potentials Glutamic Acid Biology Kynurenate Article gamma-Aminobutyric acid GABA Antagonists Glutamatergic chemistry.chemical_compound medicine Animals Picrotoxin RNA Messenger GABA Agonists gamma-Aminobutyric Acid DNA Primers Neurons Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Glutamate receptor DNA GABA receptor antagonist Adaptation Physiological Immunohistochemistry Electrophysiology Pyridazines chemistry Cats Biophysics GABAergic Female Neuroglia Neuroscience Pacinian Corpuscles medicine.drug |
| Zdroj: | The Journal of Neuroscience. 29:2695-2705 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.5974-08.2009 |
| Popis: | Pacinian corpuscles (PCs) are tactile receptors composed of a nerve ending (neurite) that is encapsulated by layers of lamellar cells. PCs are classified as primary mechanoreceptors because there is no synapse between the transductive membrane and the site of action-potential generation. These touch receptors respond in a rapidly adapting manner to sustained pressure (indentation or displacement), which until now was believed to be attributable solely to the mechanical properties of the capsule. However, evidence of positive immunoreactivity for GABA receptors on the neurite, as well as evidence for gene expression of synaptobrevin in the lamellar cells led to the hypothesis that GABAergic inhibition originating from the lamellar cells is involved in the rapid adaptation process of PCs. Electrophysiological data from isolated PCs demonstrates that, in the presence of either gabazine or picrotoxin (GABA receptor antagonists), many action potentials appear during the static portion of a sustained indentation stimulus (similar to slowly adapting receptors) and that these “static” spikes completely disappear in the presence of GABA. It was consequently hypothesized that glutamate, released by either the neurite itself or the lamellar cells, caused these action potentials. Indeed, the glutamate receptor blocker kynurenate either decreased or totally eliminated the static spikes. Together, these results suggest that GABA, emanating from the modified Schwann cells of the capsule, inhibits glutamatergic excitation during the static portion of sustained pressure, thus forming a “mechanochemical,” rather than purely mechanical, rapid adaptation response. This glial–neuronal interaction is a completely novel finding for the PC. |
| Databáze: | OpenAIRE |
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