ANTI-TUBULAR BASEMENT MEMBRANE ANTIBODIES AND GIANT CELL FORMATION IN A MODEL OF CHRONIC RENAL ALLOGRAFT REJECTION
| Autor: | Cees van Kooten, Simone A. Joosten, Hallgrimur Benediktsson, Mieneke G.A. van Dixhoorn, Maria C. Borrias, Leendert C. Paul, Vanessa J. van Ham |
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| Rok vydání: | 2004 |
| Předmět: |
Graft Rejection
Pathology medicine.medical_specialty Gene Expression Giant Cells Basement Membrane Interferon-gamma Antigen Isoantibodies medicine Animals Transplantation Homologous Basement membrane Transplantation Kidney Granuloma Base Sequence biology Histology DNA Kidney Transplantation Rats Inbred F344 Rats Disease Models Animal Kidney Tubules surgical procedures operative medicine.anatomical_structure Rats Inbred Lew Giant cell Chronic Disease Immunology biology.protein Immunohistochemistry Antibody |
| Zdroj: | Transplantation. 77:1295-1297 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000122189.53240.b9 |
| Popis: | BACKGROUND In the Fisher (F344)-to-Lewis (LEW) kidney transplantation (Tx) model of chronic rejection, antibodies reactive with tubular basement membranes have been found. We investigated whether giant cells, typical for a granulomatous inflammatory reaction, can be found during allograft rejection. METHODS F344-to-LEW renal Txs were performed, and kidneys were removed at various time points. Kidneys were analyzed by histology and immunohistochemistry. Interferon-gamma levels were measured by reverse-transcriptase polymerase chain reaction. RESULTS Multinucleated Langhans'-type giant cells were found in F344 allografts at days 21 and 30 and disappeared thereafter. Giant cells were not observed in LEW allografts. The giant cells were ED1, OX-43, and major histocompatibility complex class II positive. All grafts showed transient expression of interferon-gamma, peaking at days 7 to 14 post-Tx. CONCLUSION Granulomatous inflammation with giant cell formation occurs in the F344-to-LEW model and may represent an alloimmune response against tissue-specific antigens. |
| Databáze: | OpenAIRE |
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