Low Doses of Ionizing Radiation Enhance the Angiogenic Potential of Adipocyte Conditioned Medium

Autor: Isabel Diegues, Filomena Pina, Esmeralda Poli, José Pedro Rino, Susana Constantino Rosa Santos, F. Marques, Marta Pinto
Rok vydání: 2019
Předmět:
Vascular Endothelial Growth Factor A
Angiogenesis
Vascular Endothelial Growth Factor C
Biophysics
Neovascularization
Physiologic
Chick Embryo
Matrix metalloproteinase
Chorioallantoic Membrane
030218 nuclear medicine & medical imaging
Ionizing radiation
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Movement
In vivo
3T3-L1 Cells
Radiation
Ionizing
Adipocytes
Angiopoietin-1
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Radiology
Nuclear Medicine and imaging
Cells
Cultured
Radiation
Hepatocyte Growth Factor
Chemistry
Cell Differentiation
Dose-Response Relationship
Radiation
Vascular endothelial growth factor
Endothelial stem cell
Chorioallantoic membrane
Culture Media
Conditioned
030220 oncology & carcinogenesis
Cancer research
Hepatocyte growth factor
medicine.drug
Zdroj: Radiation Research. 192:517
ISSN: 0033-7587
DOI: 10.1667/rr15438.1
Popis: At low doses, ionizing radiation activates endothelial cells and promotes angiogenesis. However, it is still unknown if other cells may contribute to this process. In this study, the effect of low-dose ionizing radiation (LDIR) in modulating the pro-angiogenic potential of adipocytes was investigated. Adipocytes are known to secrete multiple angiogenic factors and adipokines that induce angiogenesis. In this work, a confluent monolayer of 3T3-L1 pre-adipocytes was exposed to low doses (0.1 and 0.3 Gy) and to higher doses (0.5, 0.8 and 1.0 Gy), as control. Our data show that the adipocyte-conditioned media (A-CM) from mature adipocytes differentiated from low-dose irradiated pre-adipocytes presented a higher angiogenic potential, compared to mature adipocytes differentiated from sham-irradiated control preadipocytes. The vascular endothelial growth factor (VEGF)-A levels were significantly increased in A-CM from the 0.1 Gy (P < 0.05) and 0.3 Gy (P < 0.01) experimental conditions and a significant increase was found in response to 0.3 Gy dose of radiation for VEGF-C, angiopoietin-2 (ANG-2) and hepatocyte growth factor (HGF). Moreover, 0.3 Gy dose of radiation significantly increased the expression of matrix metalloproteinase (MMP)-2 active forms. In vitro, the A-CM from the 0.1 and 0.3 Gy doses experimental conditions significantly accelerated endothelial cell migration after an in vitro wound healing assay. Importantly, in vivo, the A-CM corresponding to the 0.3 Gy experimental condition significantly induced the growth of more blood vessels towards the inoculation area in the chick embryo chorioallantoic membrane (CAM). In conclusion, this work reveals a new mechanism by which low-dose radiation might promote angiogenesis, enhancing the angiogenic potential of A-CM.
Databáze: OpenAIRE
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