Topical and intradermal delivery of PpIX precursors for photodynamic therapy with intense pulsed light on porcine skin model
| Autor: | Andrigo Barboza De Nardi, André Escobar, Michelle Barreto Requena, Vanderlei Salvador Bagnato, Rozana da Rocha Adams, Priscila Fernanda Campos de Menezes, José Dirceu Vollet Filho |
|---|---|
| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
Male
Injections Intradermal Swine Administration Topical medicine.medical_treatment Protoporphyrins Photodynamic therapy Dermatology Pharmacology Intense pulsed light Fluorescence Porcine skin 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Methyl aminolevulinate medicine Animals Distribution (pharmacology) TERAPIA A LASER Skin Photosensitizing Agents Intense Pulsed Light Therapy 030206 dentistry Photochemotherapy chemistry Homogeneous Models Animal Surgery Protoporphyrin sense organs Aminolevulinic acid Pulse light medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1435-604X 0268-8921 |
| DOI: | 10.1007/s10103-019-02771-y |
| Popis: | Made available in DSpace on 2019-10-06T15:39:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 In order to purposely decrease the time of the photodynamic therapy (PDT) sessions, this study evaluated the effects of PDT using topical and intradermal delivery of two protoporphyrin (PpIX) precursors with intense pulsed light (IPL) as irradiation source. This study was performed on porcine skin model, using an IPL commercial device (Intense Pulse Light, HKS801). IPL effect on different administration methods of two PpIX precursors (ALA and MAL) was investigated: a topical cream application and an intradermal application using a needle-free, high-pressure injection system. Fluorescence investigation showed that PpIX distribution by needle-free injection was more homogeneous than that by cream, suggesting that a shorter drug-light interval in PDT protocols is possible. The damage induced by IPL-PDT assessed by histological analysis mostly shows modifications in collagens fibers and inflammation signals, both expected for PDT. This study suggested an alternative protocol for the PDT treatment, possibility half of the incubation time and with just 3 min of irradiation, making the IPL-PDT, even more, promising for the clinical treatment. São Carlos Institute of Physics University of São Paulo (USP) Institute of Geosciences and Exact Sciences São Paulo State University (UNESP) College of Agricultural and Veterinary Sciences São Paulo State University (UNESP) Institute of Geosciences and Exact Sciences São Paulo State University (UNESP) College of Agricultural and Veterinary Sciences São Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink