The localization of the photosensitizer determines the dynamics of the secondary production of hydrogen peroxide in cell cytoplasm and mitochondria
| Autor: | Anna Orlova, Anna A. Brilkina, Natalia Y. Shilyagina, Larisa G. Klapshina, Nina N. Peskova, Vladimir V. Vodeneev, Anastasia A. Gorokhova, Anastasiya S. Nerush, Olga M Kutova, Irina V. Balalaeva |
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| Rok vydání: | 2020 |
| Předmět: |
Cytoplasm
Indoles Light medicine.medical_treatment Photosens Biophysics Photodynamic therapy Mitochondrion chemistry.chemical_compound Cell Line Tumor medicine Organometallic Compounds Humans Radiology Nuclear Medicine and imaging Photosensitizer Hydrogen peroxide chemistry.chemical_classification Reactive oxygen species Radiation Microscopy Confocal Photosensitizing Agents Radiological and Ultrasound Technology Chemistry Hydrogen Peroxide Hydrogen-Ion Concentration Mitochondria Epidermoid carcinoma |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 219 |
| ISSN: | 1873-2682 |
| Popis: | Photodynamic therapy (PDT) is based on the production of the cytotoxic reactive oxygen species (ROS) by light irradiation of a photosensitizer dye in the presence of molecular oxygen. Along with photochemical ROS production, it becomes evident that PDT induces massive secondary production of ROS which is registered long after the irradiation is completed. We created cell lines of human epidermoid carcinoma with the cytoplasmic and mitochondrial localization of protein sensor HyPer sensitive to hydrogen peroxide to compare its concentration in two cellular compartments. The lag-period between irradiation and accumulation of hydrogen peroxide in cells was registered; its duration was dose-dependent and increased up to 80 min when lowering the exposition dose from 50 to 15 J/cm2. We have shown that localization of the photosensitizer determines the spatiotemporal pattern of the cell response to PDT: secondary hydrogen peroxide accumulation in cell cytoplasm induced by photodynamic treatment with lysosome-localized phtalocyianine Photosens occurs several minutes prior to that in mitochondria; on the contrary, membranotropic arylcyanoporphyrazine dye leads to massive mitochondrial hydrogen peroxide production followed by its cytoplasmic accumulation. We hypothesize that photosensitizers with various physicochemical properties and intracellular localization can trigger different patterns not only of primary but also secondary ROS production leading to different cell fate outcomes. |
| Databáze: | OpenAIRE |
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