Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation
| Autor: | Hong Rye Kim, Seong-Lan Yu, Jong Woo Park, Seung-Yun Han, Jaeku Kang, Dong Il Jin |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Proteomics 0301 basic medicine Proteome Cell Survival Lymphocyte Activation Dephosphorylation 03 medical and health sciences Pancreatectomy Downregulation and upregulation Insulin-Secreting Cells Genetics Animals Regeneration E2F1 Phosphorylation Cells Cultured Cell Proliferation B-Lymphocytes 030102 biochemistry & molecular biology biology Cell growth Articles General Medicine Cell cycle Immunohistochemistry Carboxypeptidase Carboxypeptidase B Rats Cell biology pancreatic islet β-cells carboxypeptidase B1 030104 developmental biology post-translational modification pancreatic regeneration biology.protein Protein Processing Post-Translational |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2017.3141 |
| Popis: | A reduction in pancreatic islet β-cells leads to the onset of diabetes. Hence, the identification of the mechanisms inducing β-cell proliferation is important for developing a treatment course against the disease. It has been well established that post-translational modifications (PTMs) of proteins affect their functionality. In addition, PTMs have been suggested to play important roles in organ regeneration. Therefore, in this study, we investigated PTMs associated with pancreatic regeneration using two-dimensional electrophoresis. Four carboxypeptidase B1 (CPB1) proteins were identified at different isoelectric points, with the same molecular weight. The motif of CPB1 PTMs was identified by mass spectrophotometry, and the downregulation of CPB1 phosphorylation in pancreatectomy was confirmed. The dephosphorylation of CPB1 induced β-cell proliferation. We thus surmise that the altered PTM of CPB1 is associated with pancreatic regeneration. |
| Databáze: | OpenAIRE |
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