A photo-multiplier tube-based hybrid MRI and frequency domain fluorescence tomography system for small animal imaging
| Autor: | Ning Liu, Orhan Nalcioglu, Hao Gao, Gultekin Gulsen, Yuting Lin, Michael Ghijsen |
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| Rok vydání: | 2011 |
| Předmět: |
Photomultiplier
Fluorophore Materials science Radiological and Ultrasound Technology Phantoms Imaging business.industry Detector Inverse problem Magnetic Resonance Imaging Article Systems Integration chemistry.chemical_compound Spectrometry Fluorescence Optics Nuclear magnetic resonance chemistry Frequency domain Hybrid system Linear Models Animals Radiology Nuclear Medicine and imaging Tomography Molecular imaging business |
| Zdroj: | Physics in Medicine and Biology. 56:4731-4747 |
| ISSN: | 1361-6560 0031-9155 |
| DOI: | 10.1088/0031-9155/56/15/007 |
| Popis: | Fluorescence tomography (FT) is a promising molecular imaging technique that can spatially resolve both fluorophore concentration and lifetime parameters. However, recovered fluorophore parameters highly depend on the size and depth of the object due to the ill-posedness of the FT inverse problem. Structural a priori information from another high spatial resolution imaging modality has been demonstrated to significantly improve FT reconstruction accuracy. In this study, we have constructed a combined magnetic resonance imaging (MRI) and FT system for small animal imaging. A photo-multiplier tube (PMT) is used as the detector to acquire frequency domain FT measurements. This is the first MR-compatible time-resolved FT system that can reconstruct both fluorescence concentration and lifetime maps simultaneously. The performance of the hybrid system is evaluated with phantom studies. Two different fluorophores, Indocyanine Green (ICG) and 3-3′ Diethylthiatricarbocyanine Iodide (DTTCI), which have similar excitation and emission spectra but different lifetimes, are utilized. The fluorescence concentration and lifetime maps are both reconstructed with and without the structural a priori information obtained from MRI for comparison. We show that the hybrid system can accurately recover both fluorescence intensity and lifetime within 10% error for two 4.2 mm-diameter cylindrical objects embedded in a 38 mm-diameter cylindrical phantom when MRI structural a priori information is utilized. |
| Databáze: | OpenAIRE |
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