Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003

Autor: Ian M. Mackay, Andreas Nitsche, P. McErlean, N.T. O’Neill, Stephen B. Lambert, Katherine E. Arden, Michael D. Nissen, Theo P. Sloots, Cassandra E. Faux
Rok vydání: 2010
Předmět:
Male
Rhinovirus
Acute respiratory tract infection
Sequence Homology
medicine.disease_cause
HRV C
Epidemiology
Prevalence
Cluster Analysis
Medicine
Child
Virus discovery
Respiratory Tract Infections
Aged
80 and over
Molecular Epidemiology
0303 health sciences
education.field_of_study
Respiratory tract infections
Reverse Transcriptase Polymerase Chain Reaction
Middle Aged
3. Good health
Hospitalization
Wheeze
Infectious Diseases
Child
Preschool
RNA
Viral
Female
medicine.symptom
Adult
medicine.medical_specialty
Adolescent
Fever
Genotype
Molecular Sequence Data
Population
Asymptomatic
Article
Young Adult
03 medical and health sciences
Virology
Humans
Amino Acid Sequence
education
Aged
Respiratory Sounds
030304 developmental biology
Asthma
Picornaviridae Infections
030306 microbiology
business.industry
Infant
Newborn
Infant
Sequence Analysis
DNA
medicine.disease
Cough
Immunology
Respiratory virus epidemiology
business
Zdroj: Journal of Clinical Virology
ISSN: 1386-6532
Popis: Background Human rhinoviruses (HRVs) are associated with more acute respiratory tract infections than any other viral group yet we know little about viral diversity, epidemiology or clinical outcome resulting from infection by strains, in particular the recently identified HRVs. Objectives To determine whether HRVC-QCE was a distinct HRV-C strain, by determining its genome and prevalence, by cataloguing genomic features for strain discrimination and by observing clinical features in positive patients. Study design Novel real-time RT-PCRs and retrospective chart reviews were used to investigate a well-defined population of 1247 specimen extracts to observe the prevalence and the clinical features of each HRV-QCE positive case from an in- and out-patient pediatric, hospital-based population during 2003. An objective illness severity score was determined for each HRVC-QCE positive patient. Results Differences in overall polyprotein and VP1 binding pocket residues and the predicted presence of a cis-acting replication element in 1B defined HRVC-QCE as a novel HRV-C strain. Twelve additional HRVC-QCE detections (1.0% prevalence) occurred among infants and toddlers (1–24 months) suffering mild to moderate illness, including fever and cough, who were often hospitalized. HRVC-QCE was frequently detected in the absence of another virus and was the only virus detected in three (23% of HRVC-QCE positives) children with asthma exacerbation and in two (15%) toddlers with febrile convulsion. Conclusions HRVC-QCE is a newly identified, genetically distinct HRV strain detected in hospitalized children with a range of clinical features. HRV strains should be independently considered to ensure we do not overestimate the HRVs in asymptomatic illness.
Databáze: OpenAIRE
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