Biotransformation of Benzo[ a]pyrene by Three Rainbow Trout ( Onchorhynchus mykiss) Cell Lines and Extrapolation To Derive a Fish Bioconcentration Factor
| Autor: | Kristin Schirmer, Melanie Fischer, Julita Stadnicka-Michalak, Frederik T. Weiss, Katrin Tanneberger |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gills animal structures Bioconcentration 010501 environmental sciences 01 natural sciences Cell Line 03 medical and health sciences chemistry.chemical_compound Biotransformation In vivo Benzo(a)pyrene Environmental Chemistry Animals 0105 earth and related environmental sciences Chemistry General Chemistry 030104 developmental biology Biochemistry Cell culture Bioaccumulation Oncorhynchus mykiss Pyrene Rainbow trout |
| Zdroj: | Environmental sciencetechnology. 52(5) |
| ISSN: | 1520-5851 |
| Popis: | Permanent fish cell lines constitute a promising complement or substitute for fish in the environmental risk assessment of chemicals. We demonstrate the potential of a set of cell lines originating from rainbow trout ( Oncorhynchus mykiss) to aid in the prediction of chemical bioaccumulation in fish, using benzo[ a]pyrene (BaP) as a model chemical. We selected three cell lines from different tissues to more fully account for whole-body biotransformation in vivo: the RTL-W1 cell line, representing the liver as major site of biotransformation, and the RTgill-W1 (gill) and RTgutGC (intestine) cell lines, as important environment-organism interfaces, which likely influence chemical uptake. All three cell lines were found to effectively biotransform BaP. However, rates of in vitro clearance differed, with the RTL-W1 cell line being most efficient, followed by RTgutGC. Co-exposures with α-naphthoflavone as potent inhibitor of biotransformation, assessment of CYP1A catalytic activity, and the progression of cellular toxicity upon prolonged BaP exposure revealed that BaP is handled differently in the RTgill-W1 compared to the other two cell lines. Application of the cell-line-derived in vitro clearance rates into a physiology-based toxicokinetic model predicted a BaP bioconcentration factor (BCF) of 909-1057 compared to 920 reported for rainbow trout in vivo. |
| Databáze: | OpenAIRE |
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