Women in sports: The applicability of reference intervals for 6 commercially available testosterone immunoassays (HemSter Study)
| Autor: | Pieter Vermeersch, Ana-Maria Simundic, Anamarija Đuras, Ivanka Ostroški, Nele Peersman, Lana Ružić, Vanja Radišić Biljak, Valentina Vidranski, Tihomir Vidranski, Helena Čičak |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
030213 general clinical medicine Adolescent Clinical Biochemistry Siemens Population Physical activity Physiology 030204 cardiovascular system & hematology Young Adult 03 medical and health sciences 0302 clinical medicine Reference Values Tandem Mass Spectrometry Humans Medicine Testosterone education Exercise Immunoassay Siemens Immulite education.field_of_study Kinesiology biology Athletes business.industry Testosterone (patch) General Medicine Reference Standards biology.organism_classification Reference intervals Female business Female athletes Sport Chromatography Liquid Sports |
| Zdroj: | Clinical Biochemistry. 84:55-62 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2020.06.006 |
| Popis: | Background Testosterone levels in female athletes are increased due to their physical activity and correlate with their exercise volume. We therefore hypothesized that the reference intervals (RIs) derived from the general population are not applicable for female athletes. The aim of this study was to evaluate the applicability of the given RIs for 6 commercially available testosterone immunoassays in a group of female athletes. Methods Our study included 121 female athletes from various sporting disciplines (water polo, handball, volleyball, football, and basketball). The physical activity score was assessed by the Short Form of the International Physical Activity Questionnaire. Total testosterone was measured in serum samples by the reference LC–MS/MS method and six different immunoassays (Abbott Architect 2nd Generation Testosterone, Beckman Coulter Access Testosterone, Roche Elecsys Testosterone II, Siemens Atellica® IM Testosterone II (TSTII), Siemens IMMULITE 2000 Total Testosterone, and Snibe MAGLUMI™ Testosterone). Results There were statistically significant differences in age (P = 0.042), weight (P = 0.001), height (P < 0.001), and BMI (P < 0.001) between athletes across different sports. Their quantitative measurements of physical activity and testosterone concentration did not differ significantly between subgroups of various sports, P = 0.167 and P = 0.181, respectively. All immunoassays had a positive absolute and relative bias, in comparison with the LC–MS/MS. The manufacturer’s RI was not verified for Abbott Architect, Beckman Coulter Access, and Roche Elecsys Testosterone methods, with the highest percentage of athletes above RI for Beckman Coulter (30%). Conclusions We demonstrated that the upper reference limit provided was too low for some young female athletes. Clinical laboratories should consider the implementation of the new proposed RIs. |
| Databáze: | OpenAIRE |
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