| Autor: |
Juan-Juan, Wang, Zi-Lin, Chen, Ding-Ding, Wang, Kun-Fang, Wu, Wang-Bin, Huang, Li-Qin, Zhang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biochemical and Biophysical Research Communications. 630:92-100 |
| ISSN: |
0006-291X |
| DOI: |
10.1016/j.bbrc.2022.09.016 |
| Popis: |
We aim to investigate the potential role and underlying mechanisms of linc00174 on pyroptosis in the pathogenesis of DR.Expression patterns of linc00174, miR-26a-5p and PTEN in human retinal microvascular endothelial cells (hRMECs) were detected by quantitative real-time PCR (qRT-PCR) and Western blot, respectively. Biological functions of linc00174 on cell proliferation and pyroptosis were evaluated by CCK-8, flow cytometry, caspase-1 activity assays, respectively. Luciferase reporter assay was employed to verify the interaction between miR-26a-5p and linc00174/PTEN. Streptozotocin (STZ)-induced DR in mice was further constructed to verify the potential role of linc00174 in vivo. Hematoxylin and eosin (HE) and immunohistochemical staining were performed to assess the pathological changes and caspase-1 expression in retinal tissues.Up-regulated linc00174 and PTEN and down-regulated miR-26a-5p were uncovered in hRMECs treated with high glucose (HG). Mechanistically, linc00174 served as a sponge of miR-26a-5p to facilitate PTEN expression. Functionally, knockdown of linc00174 inhibited HG-induced pyroptosis of hRMECs via targeting miR-26a-5p. Moreover, linc00174/miR-26a-5p axis participated in HG-induced pyroptosis via PTEN/Akt signaling cascade. Further, silencing of linc00174 attenuated pyroptosis via regulating miR-26a-5p/PETN axis in DR mice.Collectively, our study reveals that linc10074 deteriorates the pathogenesis of DR via miR-26a-5p/PTEN/Akt signalling cascade, which may shed light on the discovery of potential therapeutic agents for DR treatment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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