Can crude alkaloids extract of Rhazya stricta induce apoptosis in pancreatic cancer: In vitro study?
| Autor: | Nehad A. Shaer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Chemistry Diet therapy Cancer 030204 cardiovascular system & hematology Pharmacology Rhazya stricta biology.organism_classification medicine.disease Pathology and Forensic Medicine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Apoptosis Physiology (medical) Pancreatic cancer Adjuvant therapy medicine Cytotoxic T cell MTT assay |
| Zdroj: | Pathophysiology : the official journal of the International Society for Pathophysiology. 26(1) |
| ISSN: | 0928-4680 |
| Popis: | Cancer is a complicateddisease that reveals genetic variability even among the cells within the same tumor. Pancreatic cancer is the 12 th cause of cancer deaths over the world. As a result of the incomplete recovery and the many side effects of current clinical treatment approaches, Herbal diet therapy as a single or adjuvant therapy show high significant output in cancer treatment. Our study focused on the role of the crude alkaloid extract of Rhazya stricter (R. stricta ) on pancreatic cancer cells using MTT assay. The cytotoxic effect of different concentrations of R. Strict crude alkaloid on the pancreatic cancer cells showed significant decrease in cell viability with dose dependent manner and the effect was observed at higher concentration of crude R. Stricta alkaloids. On the other hand, no significant cytotoxic effect was observed with the normal WISH cells at all R. Stricta crude alkaloid concentrations with IC50. Moving on, in AsPC-1cells under the same concentrations mRNA expression was increased by 1.5 and 6 folds with 10 and 100 μg/ Ml treatment when compared with control. Under the same experimental conditions, the anti- apoptotic marker Bcl-2 showed high significant decrease in mRNA expression in both PANC-1 and AsPC-1 pancreatic cancer cells. The present study indicated that the crude alkaloids extract of R. stricta significantly induce apoptotic cell death in pancreatic cancer cells. |
| Databáze: | OpenAIRE |
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