Development of Specific Antisera against 9-Desglycinamide-8- Arginine Vasopressin by Site-Specific Immunization
| Autor: | Jan W. van Nispen, Douwe D. Breimer, J. Coos Verhoef, Joost B. M. M. van Bree |
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| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Vasopressin Arginine Molecular Sequence Data Pharmaceutical Science Endogeny Cross Reactions Biology Pharmacokinetics Antibody Specificity Internal medicine medicine Animals Amino Acid Sequence chemistry.chemical_classification Antiserum Immune Sera Reproducibility of Results Arginine Vasopressin Endocrinology chemistry Thiol biology.protein Female Immunization Rabbits Antibody hormones hormone substitutes and hormone antagonists Conjugate |
| Zdroj: | Journal of Pharmaceutical Sciences. 80:46-49 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600800112 |
| Popis: | Four specific and sensitive antisera against 9-desglycinamide-8-arginine vasopressin (DGAVP) were obtained by means of site-specific immunization in rabbits, using a Keyhole Limpet haemocyanin-DGAVP conjugate synthesized by a specific reaction of a thiol function with a maleimide group. Compared with conventionally raised antisera, the cross-reactivity with endogenous arginine vasopressin (AVP) and vasopressin-derived fragments was substantially lower. Interassay variability was 18.1% at maximum. Possible applicability of the new antisera in pharmacokinetic studies of DGAVP with respect to blood-brain barrier transport was tested by means of a pilot study in the rat. |
| Databáze: | OpenAIRE |
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