LncRNA SNHG5 promotes the progression of osteosarcoma by sponging the miR-212-3p/SGK3 axis

Autor:	Ruihao Zhou, Feifei Zhang, Jun Sun, Shifan Lin, Xiaofeng Tang, Xiao-Bin Lv, Xiaoyong Lan, Cheng Ju, Zhiping Zhang, Kaddie Kwok Chen, Junliang Zhao
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Cancer Research lcsh:RC254-282 Metastasis Flow cytometry miR-212-3p 03 medical and health sciences 0302 clinical medicine Genetics medicine lcsh:QH573-671 SGK3 Cell proliferation Gene knockdown Osteosarcoma medicine.diagnostic_test Cell growth Chemistry lcsh:Cytology Cell invasion and migration medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis lncRNA SNHG5 Cancer research MiR-212 Signal transduction
Zdroj:	Cancer Cell International, Vol 18, Iss 1, Pp 1-13 (2018)
ISSN:	1475-2867
DOI:	10.1186/s12935-018-0641-9
Popis:	Background Long non-coding RNA (lncRNA) SNHG5 has been found to play an important role in tumors. Nevertheless, the function and mechanism of lncRNA SNHG5 in osteosarcoma (OS) remains unclear. The purpose of this study was to investigate whether lncRNA SNHG5 can regulate the occurrence and development of OS cells. Methods We performed quantitative real time PCR to detect the expression of lncRNA SNHG5 in OS cells. 143B, MG63 (knockdown) and U2OS, U2R (overexpression) cell lines were chosen for the function study of SNHG5. The effect of SNHG5, miR-212-3p, and SGK3 in OS cells was explored by MTT assays, clony formation, flow cytometry, transwell assays, wound healing assays, and cell spreading assays. Quantitative real-time PCR, Western blot analysis and luciferase assays were used to detect the interaction between lncRNA SNHG5 and miR-212-3p. Results In this study, knockdown of lncRNA SNHG5 suppressed the growth and metastasis of OS cells, whereas the overexpression of SNHG5 produced an opposite result. Mechanistically, lncRNA SNHG5 functions as a sponger against miR-212-3p and suppresses the miR-212-3p/SGK3 signaling pathway. Introduction of miR-212-3p mimics or inhibitors reverses SNHG5 overexpression or silences the exerted tumor promoting or suppressing effect. In addition, our results showed that the function of SNHG5 can be rescued by miR-212-3p and can regulate the growth and metastasis of OS cells via SGK3, the downstream target of miR-212-3p. Conclusions In summary, our study demonstrated that lncRNA SNHG5 can regulate the proliferation and metastasis of OS cells through the miR-212-3p/SGK3 axis. This axis may provide a new target for future clinical treatment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62679c453c6030bcc6a3f5f39c78a15d http://link.springer.com/article/10.1186/s12935-018-0641-9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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