N-Methyl-d-glutamate and N-methyl-l-glutamate in Scapharca broughtonii (Mollusca) and other invertebrates
| Autor: | Toratane Munegumi, Kimihiko Shibata, Atsuko Tarui, Shouji Takahashi, Ryo-hei Yamada, Yoshio Kera |
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| Rok vydání: | 2003 |
| Předmět: |
D-Aspartate Oxidase
N-Methylaspartate Time Factors Physiology Biochemistry High-performance liquid chromatography chemistry.chemical_compound Glutamates Animals Derivatization Molecular Biology Mollusca Chromatography High Pressure Liquid Oxidase test Chromatography biology Glutamate receptor Stereoisomerism biology.organism_classification chemistry NMDA receptor Ethyl chloroformate Amino Acid Oxidoreductases Chromatography Thin Layer Enantiomer |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 134:79-87 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/s1096-4959(02)00231-2 |
| Popis: | The presence of N-methyl- d -glutamate (NMDG) and N-methyl- l -glutamate (NMLG) has been demonstrated in the tissues of Scapharca broughtonii, which are known to contain N-methyl- d -aspartate (NMDA). To our knowledge, this is the first report on the natural occurrence of NMDG and the occurrence of NMLG in eukaryotes. These compounds were identified according to the following findings; (a) their derivatives with (+)- and (−)-l-(9-fluorenyl)ethyl chloroformate (FLEC) showed identical behaviors with those of authentic NMDG and NMLA, respectively, on high-performance liquid chromatography (HPLC), (b) the HPLC peak of NMDG disappeared when the extract, as well as the authentic compound, was treated with d -aspartate oxidase before derivatization, (c) they behaved identically with authentic compounds on thin-layer chromatography and differently from NMDA. Both or either of NMDG and NMLG were also detected in several mollusks and other animals. Concentrations of the enantiomers were comparable in the tissues of S. broughtonii and a few other species. |
| Databáze: | OpenAIRE |
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