Raman Spectroscopic Analysis to Detect Reduced Bone Quality after Sciatic Neurectomy in Mice

Autor: Hiromasa Miura, Yasumitsu Ishimaru, Kazunori Hino, Tadahiro Iimura, Yusuke Oshima, Sota Takanezawa, Yuuki Imai
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Osteoporosis
Pharmaceutical Science
02 engineering and technology
Matrix (biology)
Spectrum Analysis
Raman
Article
Bone and Bones
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
symbols.namesake
Mice
0302 clinical medicine
lcsh:Organic chemistry
Bone Density
Drug Discovery
medicine
Animals
collagen crosslink
bone matrix
Physical and Theoretical Chemistry
Bone mineral
mineral maturity
Principal Component Analysis
Chemistry
Organic Chemistry
Sham surgery
hydroxyapatite
030206 dentistry
Bone fracture
X-Ray Microtomography
021001 nanoscience & nanotechnology
medicine.disease
Denervation
Sciatic Nerve
osteoporosis
bone quality
sciatic neurectomy
Sciatic neurectomy
Chemistry (miscellaneous)
Raman spectroscopy
symbols
Molecular Medicine
Collagen
0210 nano-technology
Quantitative analysis (chemistry)
Biomedical engineering
Zdroj: Molecules, Vol 23, Iss 12, p 3081 (2018)
Molecules
Volume 23
Issue 12
ISSN: 1420-3049
Popis: Bone mineral density (BMD) is a commonly used diagnostic indicator for bone fracture risk in osteoporosis. Along with low BMD, bone fragility accounts for reduced bone quality in addition to low BMD, but there is no diagnostic method to directly assess the bone quality. In this study, we investigated changes in bone quality using the Raman spectroscopic technique. Sciatic neurectomy (NX) was performed in male C57/BL6J mice (NX group) as a model of disuse osteoporosis, and sham surgery was used as an experimental control (Sham group). Eight months after surgery, we acquired Raman spectral data from the anterior cortical surface of the proximal tibia. We also performed a BMD measurement and micro-CT measurement to investigate the pathogenesis of osteoporosis. Quantitative analysis based on the Raman peak intensities showed that the carbonate/phosphate ratio and the mineral/matrix ratio were significantly higher in the NX group than in the Sham group. There was direct evidence of alterations in the mineral content associated with mechanical properties of bone. To fully understand the spectral changes, we performed principal component analysis of the spectral dataset, focusing on the matrix content. In conclusion, Raman spectroscopy provides reliable information on chemical changes in both mineral and matrix contents, and it also identifies possible mechanisms of disuse osteoporosis.
Databáze: OpenAIRE
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