Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2)

Autor:	Daisuke Matsumoto, Masahiro Natsume, Hiroshi Kawaide, Yuu Aoki
Rok vydání:	2011
Předmět:	Spores Bacterial Pharmacology biology Hypha genetic processes fungi Streptomyces coelicolor information science Germicidin biology.organism_classification Spore Microbiology chemistry.chemical_compound chemistry Pyrones Drug Discovery Botany Spore germination bacteria Elongation
Zdroj:	The Journal of Antibiotics. 64:607-611
ISSN:	1881-1469 0021-8820
Popis:	Four germicidin homologs were isolated from a liquid culture of Streptomyces coelicolor A3(2). These were identified as germicidins A, B and C, and surugapyrone A (germicidin D). Absolute stereochemistry of the chiral center in germicidins A and C is determined to be S. All germicidins inhibited germination of S. coelicolor A3(2) spores above 1 μg ml(-1). S. coelicolor A3(2) spores collected from a single petri dish (9 cm i.d.) contained 5.4 μg of germicidin A (-2.7 × 10(-14) g per spore), which accounts for 2.3% of the spore extract, and contents of germicidins B, C and D were 0.2-0.8 μg. The activity of the spore extract corresponded well with the sum of the activity of each germicidin, which was estimated from the content and dose-response curve, which indicates that germicidins functions as self-germination inhibitors in S. coelicolor A3(2). Inhibitory action of germicidin A on spore germination was reversible and germicidin A inhibited not only spore germination but also hyphal elongation.
Databáze:	OpenAIRE
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