Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
| Autor: | Cliona O'Farrelly, Paul Kelly, Kieran G. Meade, Aspinas Chapwanya, Mounir Adnane |
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| Přispěvatelé: | The Algerian Ministry for High Education and Scientific Research, University of Tiaret, Algeria, Department of Agriculture, Food and the Marine, Ireland, ofarrecl-HRB-HRA_POR/2012/37, 13/S/472 |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Reducing agent Uterus Cattle Diseases Improved method Vaginal mucus 0403 veterinary science Andrology 03 medical and health sciences Postpartum medicine Animals Bicinchoninic acid assay Inflammation Uterine Diseases Cervico-vaginal mucus lcsh:Veterinary medicine General Veterinary business.industry Methodology Article Postpartum Period Biomarker 04 agricultural and veterinary sciences General Medicine medicine.disease Mucus 030104 developmental biology medicine.anatomical_structure Cervix Mucus Vagina lcsh:SF600-1100 Biomarker (medicine) Cattle Female Endometritis business Biomarkers |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 14, Iss 1, Pp 1-6 (2018) |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-018-1619-5 |
| Popis: | peer-reviewed Background In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our improved protocol results in lower protein yields but improved detection of cytokines and chemokines. Using our modified method to measure AGP in CVM we found raised levels of AGP at seven days postpartum in CVM from cows that went on to develop endometritis. Conclusion We conclude that processing CVM without reducing agents improves detection of biomarkers that reflect uterine health in cattle. We propose that measurement of AGP in CVM during the first week postpartum may identify cows at risk of developing clinical endometritis. |
| Databáze: | OpenAIRE |
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