Evaluation of sperm protamine deficiency and apoptosis in infertile men with idiopathic teratozoospermia
| Autor: | Farzaneh Fesahat, Fatemeh Yazdinejad, Fatemeh Dehghanpour, Ali Reza Talebi, Nasim Tabibnejad |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Infertility endocrine system Tapered head Sperm morphology Apoptosis Semen Biology Semen analysis Teratozoospermia Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine reproductive and urinary physiology Protamine deficiency 030219 obstetrics & reproductive medicine TUNEL assay medicine.diagnostic_test urogenital system medicine.disease Protamine Sperm 030104 developmental biology Reproductive Medicine chemistry biology.protein Original Article Chromomycin A3 |
| Zdroj: | Clinical and Experimental Reproductive Medicine |
| ISSN: | 2233-8241 2233-8233 |
| DOI: | 10.5653/cerm.2017.44.2.73 |
| Popis: | Objective Sperm morphology plays an important role in infertility, especially in cases of defects in the heads of spermatozoa. Tapered-head or elongated-head spermatozoa are examples of morphological abnormalities. The aim of this study was to compare the semen parameters, levels of protamine deficiency, and frequency of apoptosis between patients with normozoospermia and those with teratozoospermia with tapered-head spermatozoa. Methods Fifty-two semen samples (27 patients with tapered-head sperm and 25 fertile men) were collected and semen analysis was performed according to the World Health Organization criteria for each sample. Protamine deficiency and the percentage of apoptotic spermatozoa were evaluated using chromomycin A3 (CMA3) staining and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assays, respectively. Results Sperm concentration, motility, and normal morphology in the tapered-head spermatozoa (cases) were significantly lower than in the normozoospermic samples (controls). CMA3-reactive spermatozoa (CMA3+) in the case group were more common than in the controls. Apoptotic spermatozoa (TUNEL-positive) were significantly more common in the cases than in the controls. Conclusion This analysis showed that tapered-head spermatozoa contained abnormal chromatin packaging and exhibited a high rate of apoptosis, which can be considered to be an important reason for the impaired fertility potential in teratozoospermic patients with tapered-head spermatozoa. |
| Databáze: | OpenAIRE |
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