Association between SREBP-1 gene expression in mammary gland and milk fat yield in Sarda breed sheep☆
| Autor: | Vincenzo Carcangiu, Maria Consuelo Mura, Cinzia Daga, Sebastiano Luridiana, Sara Bodano, Giovanni Sanna, Maria Luisa Diaz, Giovanni Cosso |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Milk somatic cell Mammary gland Biology Article SREBP-1 gene expression chemistry.chemical_compound Animal science Internal medicine Lactation Gene expression Genetics medicine Lactose Genetics (clinical) Domestic sheep reproduction food and beverages Sarda sheep biology.organism_classification Breed RNA extraction Early lactation Milk fat yield medicine.anatomical_structure Endocrinology chemistry Sarda |
| Zdroj: | Meta Gene |
| ISSN: | 2214-5400 |
| Popis: | Summary The aim of this study was to examine the expression patterns of SREBP-1 gene in milk somatic cells and its association with milk fat yield during early lactation in Sarda breed sheep. A sample of 20 Sarda ewes, aged between 4 and 5 years, in their third to fourth lactation were chosen. From each ewe 28 days after lambing milk yield was measured, and a 160 ml milk sample for the RNA extraction and to test somatic cells count and lactose, fat and protein contents were collected. From the obtained RNA, total cDNA was synthesized and the quantitative PCR was performed. The fat, proteins and lactose content showed many differences among the animals, but these variations were no correlated with the milk yield. The SREBP-1 gene expression resulted higher in the high milk fat producing ewes. The correlation analysis showed that the SREBP-1 expression level is directly related to the amount of milk fat (g/die) (P Highlights • We examined the SREBP-1 gene expression in milk somatic cell in the sheep. • The expression of this gene is related with fat yield (g/die). • Instead, no relationship was found between gene expression and milk fat concentration. • A high SREBP-1 gene expression is necessary for the higher milk fat yield. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|