DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target
| Autor: | Yasunori Machida, Toshiharu Ohba, Yasushi Yoshioka, Chiyoko Machida |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Base pair Molecular Sequence Data Plant Science Biology Transfection law.invention Sequence-tagged site chemistry.chemical_compound Transformation Genetic law Tobacco Genetics Escherichia coli Cloning Molecular Polymerase chain reaction Recombination Genetic Base Sequence Inverse polymerase chain reaction Nucleic acid sequence Cell Biology Gene rearrangement Molecular biology Plants Toxic chemistry Agrobacterium tumefaciens In vitro recombination DNA |
| Zdroj: | The Plant journal : for cell and molecular biology. 7(1) |
| ISSN: | 0960-7412 |
| Popis: | Transferred DNA (T-DNA) of the tumor-inducing (Ti) plasmid is transferred from Agrobacterium tumefaciens to plant cells and is stably integrated into the plant nuclear genome. By the inverse polymerase chain reaction DNA fragments were amplified that contained the T-DNA/plant DNA junctions from the total DNA of a transgenic tobacco plant that had a single copy of the T-DNA in a repetitive region of its genome. A DNA fragment containing the target site was amplified from the total DNA of non-transformed tobacco by the polymerase chain reaction using high-stringency conditions. Comparison of the nucleotide sequence of the target site with those of the T-DNA/plant DNA junctions revealed that various duplications of short stretches of nucleotide sequences around the target and in the incoming T-DNA had accompanied the integration of the T-DNA. A deletion of 16 bp at the target site was also found and the target site was similar, in terms of nucleotide sequence, to regions around the breakpoints of the T-DNA. This finding provides a clear example of the occurrence of complex rearrangements during the integration of T-DNA. |
| Databáze: | OpenAIRE |
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