The beta-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex
| Autor: | Chun-Ju Tai, Chien-Hsun Huang, Fwu-Ling Lee |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Biology Microbiology Saccharomyces DNA Ribosomal Fungal Proteins Phylogenetics Tubulin parasitic diseases DNA Fungal Molecular Biology Phylogeny Genetics Phylogenetic tree Fungal genetics Genes rRNA RNA Fungal General Medicine Sequence Analysis DNA Ribosomal RNA biology.organism_classification DNA Fingerprinting DNA profiling RNA Ribosomal Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Antonie van Leeuwenhoek. 95(2) |
| ISSN: | 1572-9699 |
| Popis: | The Saccharomyces sensu stricto complex comprises seven very closely related species. In this study, we compared the use of two different phylogenetic markers, the 26S rDNA and beta-tubulin genes, for discriminating phylogenetic relationships among Saccharomyces sensu stricto strains using sequencing as well as RFLP methods. The average sequence similarity for the beta-tubulin gene (90.0%) among seven strains was significantly less than that for 26S rDNA (98.6%). This result demonstrates that beta-tubulin gene sequences provided higher resolution than 26S rDNA sequences. Species-specific restriction profiles of the Saccharomyces strains were obtained by cutting them with the Tsp509I enzyme. Our data indicate that phylogenetic relationships between these strains are best resolved using sequencing or RFLP analysis of the beta-tubulin gene. |
| Databáze: | OpenAIRE |
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