Chloroplast biogenesis: Quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry

Autor:	Shi-Ming Wu, Joseph M. Mayasich, Constantin A. Rebeiz
Rok vydání:	1989
Předmět:	Chlorophyll Chloroplasts Chromatography Chlorophyllides Biophysics Analytical chemistry Ether Cell Biology Biochemistry Quantitative determination Chloroplast Hexane chemistry.chemical_compound Spectrometry Fluorescence chemistry Molecular Biology Mathematics Biogenesis
Zdroj:	Analytical Biochemistry. 178:294-300
ISSN:	0003-2697
DOI:	10.1016/0003-2697(89)90641-6
Popis:	Simultaneous equations for the determination of monovinyl (MV) and divinyl (DV) Chl a and b by spectrofluorometry in unsegregated mixtures of these tetrapyrroles were derived. The same equations can also be used for the quantitative determination of MV and DV chlorophyllide (Chlide) a and b in unpurified mixtures, after extraction of the Chls in hexane. The equations used differences in the Soret excitation maxima of these tetrapyrroles in ether at 77°K, in order to correct the Soret excitation overlap between MV and DV Chl(ide) a and between MV and DV Chl(ide) b.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6259fa4a63200579eda96717ef5d5bc6 https://doi.org/10.1016/0003-2697(89)90641-6 Zobrazit plný text záznamu