Comparison of the modified polyacrylamide gradient gel electrophoresis and high-performance liquid chromatography methods in determining LDL size
| Autor: | Murat Cihan, Hideko Tsukamoto, Muhittin Serdar, M. Kemal Erbil |
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| Přispěvatelé: | Acibadem University Dspace |
| Rok vydání: | 2022 |
| Předmět: |
aterosklerozis
yuksek-performansli sivi kromatografisi polyacrylamide gradient gel electrophoresis Biochemistry (medical) Clinical Biochemistry Biochemistry LDL size koroner arter hastaligi LDL boyutu poliakrilamid gradyent jel elektroforezi atherosclerosis high-performance liquid chromatography Molecular Biology coronary artery disease |
| Popis: | Objectives In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density lipoprotein (LDL) size. Methods Blood specimens were obtained from eighty-seven volunteers. We measured LDL size using the PAGGE method and HPLC method with total cholesterol (TC) and triglyceride (TG) peaks. In the PAGGE method, Coomassie Brilliant Blue (CBB) staining was used instead of Sudan black staining, unlike the original method. The relationship between PAGGE and HPLC methods was evaluated by Pearson correlation test and Passing-Bablok regression analysis. Agreement between them was evaluated by Kappa analysis and Bland-Altman plots. Results Statistically significant correlation was found between the LDL size with PAGGE and HPLC methods under the cholesterol curve (HPLC-TC) (r=0.924, p Conclusions The PAGGE method can be a reliable method for measuring LDL size. HPLC method under cholesterol and triglyceride peaks may be used in clinical practice interchangeably, but clinical decision limits should be different. In addition, our study demonstrated that measurement methods for LDL size could be simplified with several modifications. |
| Databáze: | OpenAIRE |
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