Establishment of New Highly Insulin-Sensitive Cell Lines and Screening of Compounds to Facilitate Glucose Consumption
| Autor: | Katsuichi Sakano, Kenji Hayata, Shigeyuki Nishinaka |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
BALB 3T3 Cells
medicine.medical_treatment Glucose uptake Cell Separation Ion Channels Mitochondrial Proteins Small Molecule Libraries Mice Insulin resistance medicine Animals Hypoglycemic Agents Insulin Uncoupling Protein 3 Protein Kinase Inhibitors Pharmacology Muscle Cells Glucose Transporter Type 4 Dose-Response Relationship Drug biology lcsh:RM1-950 medicine.disease Receptor Insulin Insulin receptor Glucose lcsh:Therapeutics. Pharmacology Biochemistry Cell culture biology.protein Molecular Medicine Insulin Resistance Proto-Oncogene Proteins c-akt C2C12 GLUT4 |
| Zdroj: | Journal of Pharmacological Sciences, Vol 108, Iss 3, Pp 348-354 (2008) |
| ISSN: | 1347-8648 1347-8613 |
| Popis: | To obtain compounds that promote glucose uptake in muscle cells, the novel cell lines A31-IS derived from Balb/c 3T3 A31 and C2C12-IS from mouse myoblast C2C12 were established. In both cell lines, glucose consumption was induced by insulin and suppressed by the addition of Akt-activating kinase inhibitor. The A31-IS cells highly express the insulin receptor β chains, Glut4, and uncoupling protein-3, as compared to the parent Balb /c 3T3 A31 cells, and C2C12-IS cells highly express the insulin receptor β chain as compared to its parent cell line. Using A31-IS cells, we screened our library compounds and obtained three compounds, DF-4394, DF-4451, and DG-5451. These compounds dose-dependently promoted glucose consumption in A31-IS cells and facilitated [3H]-2-deoxyglucose uptake in differentiated C2C12-IS cells. The compounds that we obtained from the library screening will be good candidates for improving insulin resistance in muscle cells. Keywords:: skeletal muscle cell, insulin, glucose uptake |
| Databáze: | OpenAIRE |
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