Development of an Integrated Optical Sensor for Determination of β-Hydroxybutyrate Within the Microplatform
| Autor: | Utku Devamoglu, Irem Duman, Ecem Saygili, Ozlem Yesil-Celiktas |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Optics and Photonics Arduino microcontroller Point-of-care tests Bioengineering Alcohol Urine 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Absorbance chemistry.chemical_compound Health problems 010608 biotechnology Acetone Humans Molecular Biology Detection limit Chromatography 3-Hydroxybutyric Acid 010405 organic chemistry General Medicine 0104 chemical sciences chemistry Point-of-Care Testing Optical sensor Ketone bodies Microplatform Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 193(9) |
| ISSN: | 1559-0291 |
| Popis: | Ketone bodies (acetoacetate, beta-hydroxybutyrate (?HB), acetone) are generated as a result of fatty acid oxidation in the liver and exist at low concentrations in urine and blood. Elevated concentrations can indicate health problems such as diabetes, childhood hypoglycemia, alcohol, or salicylate poisoning. Development of portable and cost-effective bedside point-of-care (POC) tests to detect such compounds can help to reduce the risk of disease progression. In this study, ?HB was chosen as a model molecule for developing an optical sensor–integrated microplatform. Prior to sensor optimization, ?HB levels were measured at a concentration range of 0.02 and 0.1 mM spectrophotometrically, which is far below the reported elevated ranges of 1–2 mM and resulting absorbance changes were converted into an Arduino microcontroller code for the correlation. Measurements performed with the designed integrated microplatform were found significant. Integrated microplatform was verified with the benchtop spectrophotometer. Measurements between 0.02 and 0.1 mM substrate concentration were found highly sensitive with “y = 0.7347x + 0.00184” with R2 value of 0.9796, and the limit of detection was determined as 0.02 mM. Based on these results, the proposed system will allow on-site and early intervention. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. 1919B011800241 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK The study was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) 2209/A University Students Research Project Support Program (1919B011800241). The authors are grateful to Armagan Ergun for his contributions to PMMA mold production. E.S gratefully acknowledges the TUBITAK 2211-A National Graduate Scholarship Program. |
| Databáze: | OpenAIRE |
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