Significance of -1082A/G polymorphism of IL10 gene for progression of colorectal cancer and IL-10 expression
| Autor: | Spaska Stanilova, Tashko S. Deliysky, Noyko Stanilov, Lyuba Miteva |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Oncology
medicine.medical_specialty Genotype Colorectal cancer Gene Expression Biology Bioinformatics Polymorphism Single Nucleotide Gene Frequency Internal medicine Gene expression Genetic predisposition medicine Odds Ratio Humans Allele Neoplasm Metastasis Allele frequency Alleles Aged Neoplasm Staging Aged 80 and over Case-control study General Medicine Odds ratio Middle Aged medicine.disease Prognosis Interleukin-10 Case-Control Studies Disease Progression Neoplasm Grading Colorectal Neoplasms |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(12) |
| ISSN: | 1423-0380 |
| Popis: | The role of functional polymorphism within IL10 (rs1800896) in colorectal cancer (CRC) still remains elusive. The aim of present study was to investigate the significance of -1082A/G polymorphism in IL10 on CRC risk, progression, and overall survival in a cohort of Bulgarian patients. Also, a functional role of this polymorphism on systemic and local level of IL10 mRNA quantity and serum IL-10 level was explored. A group of 119 patients with sporadic CRC and 154 age-sex-matched controls were genotyped by allele-specific PCR. The quantification of mRNA and serum IL-10 levels was performed by real-time PCR and ELISA assays, respectively. The genotype and allelic frequency among cases and controls was similar. However, we observed significant elevation of G-allele and GG-genotype frequencies among advanced CRC. G-allele was overrepresented in advanced CRC patients (49 %) compared to early CRC (35 %) with OR = 1.77; 95%CI 1.018 ÷ 3.083; P = 0.031. A significant upregulated expression of IL10 mRNA was observed among AG/GG-genotypes in tumor tissue compared to homozygous AA-genotype (RQ value 68.3 vs. 6.68; P = 0.0062). Also, GG-genotype of -1082A/G polymorphism in IL10 was positively associated with higher serum IL-10 among early CRC patients and controls, in contrast to advanced cases. Although, investigated polymorphism in IL10 has no significant impact of overall survival among Bulgarian CRC patients, we found a significant relationship of high pre-operative serum level of IL-10 with poor survival of CRC (P = 0.023). Our findings indicate a significant impact of -1082A/G polymorphism of IL10 on CRC progression, rather than genetic predisposition and prognosis of CRC. |
| Databáze: | OpenAIRE |
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