A Nanodiamond-peptide Bioconjugate for Fluorescence and ODMR Microscopy of a Single Actin Filament
| Autor: | Ryotaro Tanabe, Ryuji Igarashi, Masahiro Shirakawa, Takuya Genjo, Shingo Sotoma |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Optics and Photonics
Nitrogen Phalloidine Biocompatible Materials Nanotechnology macromolecular substances 02 engineering and technology 01 natural sciences Fluorescence Nanodiamonds Analytical Chemistry Protein filament 0103 physical sciences Microscopy Fluorescence microscope Animals Molecule Muscle Skeletal 010306 general physics Nanodiamond Cytoskeleton Actin Rhodamines Chemistry Colocalization 021001 nanoscience & nanotechnology Actins Biomechanical Phenomena Actin Cytoskeleton Microscopy Fluorescence Biophysics Rabbits Peptides 0210 nano-technology Protein Binding |
| Zdroj: | Analytical Sciences. 32:1165-1170 |
| ISSN: | 1348-2246 0910-6340 |
| DOI: | 10.2116/analsci.32.1165 |
| Popis: | Recently, the importance of conformational changes in actin filaments induced by mechanical stimulation of a cell has been increasingly recognized, especially in terms of mechanobiology. Despite its fundamental importance, however, long-term observation of a single actin filament by fluorescent microscopy has been difficult because of the low photostability of traditional fluorescent molecules. This paper reports a novel molecular labeling system for actin filaments using fluorescent nanodiamond (ND) particles harboring nitrogen-vacancy centers; ND has flexible chemical modifiability, extremely high photostability and biocompatibility, and provides a variety of physical information quantitatively via optically detected magnetic resonance (ODMR) measurements. We performed the chemical surface modification of an ND with the actin filament-specific binding peptide Lifeact and observed colocalization of pure Lifeact-modified ND and actin filaments by the ODMR selective imaging protocol, suggesting the capability of long-term observation and quantitative analysis of a single molecule by using an ND particle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink