Low Prevalence of Leptospira Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand
| Autor: | Janjira Thaipadungpanit, Surachai Leepitakrat, Elizabeth Wanja, Federico Costa, Patrick W. McCardle, Anthony L. Schuster, Taweesak Monkanna, B Katherine Poole-Smith, Panadda Krairojananan |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine carriage Leptospira L. wolffii rodent Bandicota indica 16S rRNA gene Thailand Rodent animal diseases 030231 tropical medicine 030106 microbiology lcsh:Medicine Biology Article 03 medical and health sciences 0302 clinical medicine biology.animal medicine General Immunology and Microbiology Incidence (epidemiology) lcsh:R Public Health Environmental and Occupational Health 16S ribosomal RNA biology.organism_classification medicine.disease bacterial infections and mycoses Leptospirosis Infectious Diseases Carriage Lower prevalence bacteria |
| Zdroj: | Tropical Medicine and Infectious Disease; Volume 5; Issue 4; Pages: 154 Tropical Medicine and Infectious Disease, Vol 5, Iss 154, p 154 (2020) Tropical Medicine and Infectious Disease |
| ISSN: | 2414-6366 |
| DOI: | 10.3390/tropicalmed5040154 |
| Popis: | Leptospirosis is a neglected zoonotic disease affecting mostly the world's tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and carriage rate of pathogenic Leptospira in rodent reservoirs to leptospirosis incidence, we surveyed rodents, between 2011 and 2012, in four provinces in northeastern Thailand with the highest incidence rates of human leptospirosis cases. We used lipL32 real-time PCR to detect pathogenic Leptospira in rodent kidneys, partial 16S rRNA gene sequencing to classify the infecting Leptospira species, and whole 16S rDNA sequencing to classify species of isolated Leptospira. Overall prevalence of Leptospira infection was 3.6% (18/495). Among infected rodents, Bandicotaindica (14.3%), Rattusexulans (3.6%), and R. rattus (3.2%) had renal carriage. We identified two pathogenic Leptospira species: L. interrogans (n = 15) and L. borgpetersenii (n = 3). In addition, an L. wolffii (LS0914U) isolate was recovered from the urine of B. indica. Leptospira infection was more prevalent in low density rodent populations, such as B. indica. In contrast, there was a lower prevalence of Leptospira infection in high density rodent populations of R. exulans and R. rattus. |
| Databáze: | OpenAIRE |
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