Effects of shearing stress on aortic histamine synthesis
| Autor: | Theodore M. Hollis, Ronald A. Ferrone |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
medicine.medical_specialty Endothelium Carboxy-Lyases Clinical Biochemistry Aorta Thoracic In Vitro Techniques Pathology and Forensic Medicine Histamine synthesis chemistry.chemical_compound Internal medicine medicine.artery medicine Shear stress Animals Thoracic aorta Histidine Molecular Biology Histological examination Chemistry Histidine decarboxylase Perfusion Endocrinology medicine.anatomical_structure Anesthesia Rabbits Blood Flow Velocity Histamine |
| Zdroj: | Experimental and Molecular Pathology. 20:1-10 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/0014-4800(74)90038-0 |
| Popis: | The potential of elevated shearing stresses imposed on the endothelial surface of rabbit thoracic aorta to increase aortic histamine synthesis through activation of aortic histidine decarboxylase (HD) has been examined. Using a Tyrodes-glycerine solution (37°C, pH 7.4, 3.5 centipoise) as the perfusion solution, aortas were subjected to shearing stresses of 8 (control), 27, and 800 dynes/cm 2 , respectively. Additional nontreated and incubated controls were also used. The aortic HD activity, expressed as histamine forming capacity (HFC), for the three treatment groups were (in dpm/mg protein) 302 ± 10, 1537 ± 26, and 969 ± 16, respectively. Histological examination of similarly treated segments revealed no endothelium present in the 800 dynes/cm 2 stressed group. These data indicate that aortic histamine synthesis increased approximately 5-fold in response to a 3 1 2 - fold increase in shear exposure and that over 50% of the increase in histamine synthesis occurred in the endothelial monolayer. These data have been discussed both in relation to the role of the endothelium in regulation of, and the effects of histamine on, vascular wall permeability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|