RNA-Seq analysis for assessing the early response to DSP toxins in Mytilus galloprovincialis digestive gland and gill
| Autor: | Josefina Méndez, M. Luisa Martínez Martínez, María Verónica Prego-Faraldo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gill Gills DSP toxins Bivalves DNA Complementary animal structures Health Toxicology and Mutagenesis mussel Resistance lcsh:Medicine RNA-Seq Biology bivalves Toxicology Article Transcriptome resistance 03 medical and health sciences medicine Defense Animals Shellfish Poisoning Toxins Biological Mytilus cDNA library Sequence Analysis RNA lcsh:R fungi Immunity Mussel medicine.disease biology.organism_classification immunity Shellfish poisoning defense Gastrointestinal Tract qPCR 030104 developmental biology Metabolism Biochemistry Dinoflagellida Diarrhetic shellfish poisoning metabolism |
| Zdroj: | Toxins RUC. Repositorio da Universidade da Coruña instname Volume 10 Issue 10 Toxins, Vol 10, Iss 10, p 417 (2018) |
| Popis: | [Abstract] The harmful effects of diarrhetic shellfish poisoning (DSP) toxins on mammalian cell lines have been widely assessed. Studies in bivalves suggest that mussels display a resistance to the cytogenotoxic effects of DSP toxins. Further, it seems that the bigger the exposure, the more resistant mussels become. To elucidate the early genetic response of mussels against these toxins, the digestive gland and the gill transcriptomes of Mytilus galloprovincialis after Prorocentrum lima exposure (100,000 cells/L, 48 h) were de novo assembled based on the sequencing of 8 cDNA libraries obtained using an Illumina HiSeq 2000 platform. The assembly provided 95,702 contigs. A total of 2286 and 4523 differentially expressed transcripts were obtained in the digestive gland and the gill, respectively, indicating tissue-specific transcriptome responses. These transcripts were annotated and functionally enriched, showing 44 and 60 significant Pfam families in the digestive gland and the gill, respectively. Quantitative PCR (qPCR) was performed to validate the differential expression patterns of several genes related to lipid and carbohydrate metabolism, energy production, genome integrity and defense, suggesting their participation in the protective mechanism. This work provides knowledge of the early response against DSP toxins in the mussel M. galloprovincialis and useful information for further research on the molecular mechanisms of the bivalve resistance to these toxins. Ministerio de Economía y Competitividad; AGL2012-30897 |
| Databáze: | OpenAIRE |
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