A new assay for cell death
| Autor: | Igor P. Beletsky, S.R. Umansky |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Cell Membrane Permeability Cell Survival T-Lymphocytes Cytological Techniques Immunology Cell Population Digitonin Biology Automation Micromanipulation chemistry.chemical_compound Ethidium Tumor Cells Cultured medicine Animals Immunology and Allergy Cytotoxic T cell Viability assay Cytotoxicity education education.field_of_study Tumor Necrosis Factor-alpha Rats Inbred Strains Rats Staining Spectrometry Fluorescence medicine.anatomical_structure Biochemistry chemistry Cell culture Ethidium bromide |
| Zdroj: | Journal of Immunological Methods. 134:201-205 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(90)90381-5 |
| Popis: | A new assay for the evaluation of cell viability is described. It is based on the staining of dead cells and subsequently the whole cell population with ethidium bromide (EtBr). The method makes it possible to measure cytotoxic and cytostatic effects simultaneously and cell cultures grown both in suspension as well as by adherence may be assessed. The advantages and disadvantages of this assay are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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