Production of anti-epithelial antibodies and acantholysis by vaccination with an anti-idiotypic antibody, mimicking desmoglein 1
| Autor: | Ernesto Alvarado-Flores, Rafael Herrera-Esparza, Erendira Lopez-Robles, Esperanza Avalos-Díaz |
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| Rok vydání: | 2007 |
| Předmět: |
Idiotype
Dermatology Biochemistry Desmoglein Epitope Immunoglobulin G Immunoglobulin Fab Fragments Mice Antibody Specificity medicine Animals Molecular Biology Autoantibodies Mice Inbred BALB C Vaccines integumentary system biology business.industry Desmoglein 1 Acantholysis Vaccination Epithelial Cells medicine.disease Virology Antibodies Anti-Idiotypic Disease Models Animal Pemphigus Immunization Immunology biology.protein Rabbits Antibody business |
| Zdroj: | Experimental Dermatology. :071024173226001 |
| ISSN: | 1600-0625 0906-6705 |
| Popis: | Theoretically, the immunization of experimental animals with an anti-idiotype antibody may elicit antibodies that recognize epitopes like the original idiotype; this is archived via internal images. Using this strategy, we attempted to produce anti-epithelial antibodies in Balb/c mice immunized with a pemphigus anti-idiotypic determinant. First, when an anti-idiotype antibody was produced in rabbits by immunization with pemphigus immunoglobulin G (IgG), the anti-idiotypic activity was tested successfully. The anti-idiotype IgG was digested with pepsin and purified by gel filtration chromatography to obtain F(ab')(2) fragments, which were used to immunize Balb/c mice. A control group was immunized with normal IgG. The experimental animals immunized with anti-idiotype F(ab')(2) fragments developed anti-epithelial antibodies in the following two months. The elicited antibodies had anti-desmoglein 1 specificity. Additionally, the skin biopsies of these animals exhibited antibody deposition along intercellular spaces of epidermis, and 25% of them developed blisters. Sera and skin biopsies of control Balb/c mice group were negative. In conclusion, the immunization with pemphigus anti-idiotype antibody may elicit anti-epithelial antibodies via internal images. This experimental approach can be used to understand the pathogenic mechanisms of pemphigus. |
| Databáze: | OpenAIRE |
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