Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation

Autor:	Yamashita, Teruyoshi, Kamiya, Daisuke, Kawaraichi, Nami, Toyokawa, Harufu, Akaboshi, Teppei, Ikeya, Makoto, Toyooka, Yayoi
Rok vydání:	2021
Předmět:	QH301-705.5 Neural Crest SOXE Transcription Factors embryonic structures Induced Pluripotent Stem Cells Gene Expression Regulation Developmental Humans Cell Differentiation Cell Biology General Medicine Biology (General) Developmental Biology
Zdroj:	Stem Cell Research, Vol 60, Iss, Pp 102696-(2022)
ISSN:	1876-7753
Popis:	SOX10 (SRY-box transcription factor 10) is not only a definitive molecular marker of neural crest cells (NCCs) but also an essential transcription factor for the differentiation of NCCs in vertebrate embryogenesis. Here, we report the establishment of a human SOX10 knock-in reporter iPSC line (SOX10-tdT) by CRISPR/Cas9-mediated homologous recombination, in which the expression of SOX10 can be monitored as tdTomato fluorescence. This iPSC line can provide a useful tool to model the differentiation process of human NCCs in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::623e522197d87472272fb86b0973cbc5 https://pubmed.ncbi.nlm.nih.gov/35144211 Zobrazit plný text záznamu