Pulsed interleaved excitation fluorescence spectroscopy with a supercontinuum source
| Autor: | Emmanuel Margeat, Linnea Olofsson |
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| Rok vydání: | 2013 |
| Předmět: |
Photon
Materials science business.industry Physics::Optics Equipment Design Laser Atomic and Molecular Physics and Optics Photon counting Fluorescence spectroscopy Supercontinuum law.invention Equipment Failure Analysis Optics law Picosecond Fluorescence Resonance Energy Transfer business Spectroscopy Excitation Lighting |
| Zdroj: | Optics express. 21(3) |
| ISSN: | 1094-4087 |
| Popis: | Pulsed Interleaved Excitation (PIE) improves fluorescence cross-correlation spectroscopy (FCCS) and single pair Forster Resonance Energy Transfer (spFRET) measurements, by correlating each detected photon to the excitation source that generated it. It relies on the interleaving of two picosecond laser sources and time correlated single photon counting (TCSPC) detection. Here, we present an optical configuration based on a commercial supercontinuum laser, which generates multicoulour interleaved picosecond pulses with arbitrary spacing and wavelengths within the visible spectrum. This simple, yet robust configuration can be used as a versatile source for PIE experiments, as an alternative to an array of picosecond lasers and drivers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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