MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles

Autor: Alexia Grandjean-Laquerriere, Edouard Jallot, Patrice Laquerriere, Moncef Guenounou, Patrick Frayssinet, Salima Addadi-Rebbah, Dominique Laurent-Maquin
Přispěvatelé: Interfaces biomatériaux/tissus hôtes, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Immunologie et de Biotechnologies (LIB), Université de Reims Champagne-Ardenne (URCA)-IFR 53, Université de Reims Champagne-Ardenne (URCA)-UFR PHARMACIE, Médicaments : Dynamique Intracellulaire et Architecture Nucléaire (MéDIAN), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Corpusculaire - Clermont-Ferrand (LPC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), LE GAILLARD (GAILLARD), LE GAILLARD, Laurent-Maquin, Dominique
Rok vydání: 2004
Předmět:
Physical characteristics
Regulator
02 engineering and technology
MESH: Base Sequence
Matrix (biology)
Matrix metalloproteinase
MESH: Gelatinase B
MESH: Monocytes
MESH: Gelatinase A
Monocytes
MESH: Reverse Transcriptase Polymerase Chain Reaction
Macrophage
chemistry.chemical_classification
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
021001 nanoscience & nanotechnology
Cell biology
Membrane
Matrix Metalloproteinase 9
Biochemistry
Mechanics of Materials
Human monocytes
Matrix Metalloproteinase 2
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
MESH: DNA Primers
Materials science
Biophysics
Tissue inhibitors of metalloproteinase
Bioengineering
Matrix Metalloproteinase Inhibitors
Biomaterials
03 medical and health sciences
Humans
DNA Primers
030304 developmental biology
[SDV.IB] Life Sciences [q-bio]/Bioengineering
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
MESH: Humans
Base Sequence
In vitro
MESH: Durapatit
Durapatite
MESH: Tissue Inhibitor of Metalloproteinase-2
Enzyme
MESH: Tissue Inhibitor of Metalloproteinase-1
chemistry
Ceramics and Composites
Hydroxyapatite particles
MESH: Durapatite
Zdroj: Biomaterials
Biomaterials, 2004, 25 (13), pp.2515-24
Biomaterials, Elsevier, 2004, 25 (13), pp.2515-24
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2003.09.034
Popis: DOI : 10.1016/j.biomaterials.2003.09.034; After calcium-phosphates biomaterials based implantation like hydroxyapatite (HA) coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases (MMPs), which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases (TIMPs) are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their respective inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1β release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1β.
Databáze: OpenAIRE
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